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Minimal contraction for tissue-engineered skin substitutes when matured at the air–liquid interface

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bul.description.provenanceeb spbfr
bul.rights.dateAccepPubl2013-06-03fr
bul.rights.periodeEmbargoforeverfr
bul.rights.raisonEmbargoInfiniPour que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de Journal of tissue engineering and regenerative medicine, il faudrait déposer la version acceptée pour publication, incluant toutes les modifications demandées, mais sans la mise en page de la revue. Pour ce faire, effectuez une demande de modification à l’aide de la liste des dépôts diffusés à partir du tableau de suivi.fr
bul.rights.typeDatedatePublicationfr
dc.audience.peerreview1fr
dc.contributor.authorGermain, Lucie
dc.contributor.authorLarouche, Danielle
dc.contributor.authorAuger, François A.
dc.contributor.authorMarcoux, Hugo-Bastien
dc.contributor.authorGauvin, Robert
dc.contributor.authorGuignard, Rina
dc.date.accessioned2018-02-02T13:40:09Z
dc.date.available9999-12-31
dc.date.issued2013-06-03
dc.description.abstractThe structural stability of skin substitutes is critical to avoid aesthetic and functional problems after grafting, such as contractures and hypertrophic scars. The present study was designed to assess the production steps having an influence on the contractile behaviour of the tissue-engineered skin made by the self-assembly approach, where keratinocytes are cultured on tissue-engineered dermis comprised of fibroblasts and the endogenous extracellular matrix they organized. Thus, different aspects were investigated, such as the assembly method of the engineered dermis (various sizes and anchoring designs) and the impact of epithelial cell differentiation (culture submerged in the medium or at the air–liquid interface). To evaluate the structural stability at the end of the production, the substitutes were detached from their anchorages and deposited on a soft substrate, and contraction was monitored over 1 week. Collected data were analysed using a mathematical model to characterize contraction. We observed that the presence of a differentiated epidermis significantly reduced the amount of contraction experienced by the engineered tissues, independently of the assembly method used for their production. When the epidermis was terminally differentiated, the average contraction was only 24 4% and most of the contraction occurred within the first 12 h following deposition on the substrate. This is 2.2-fold less compared to when the epidermis was cultured under the submerged condition, or when tissue-engineered dermis was not overlaid with epithelial cells. This study highlights that the maturation at the air–liquid interface is a critical step in the reconstruction of a tissue engineered skin that possesses high structural stabilityfr
dc.identifier.doi10.1002/term.543fr
dc.identifier.issn1932-6254fr
dc.identifier.pubmed22328243fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/16838
dc.languageengfr
dc.publisherJohn Wiley & Sonsfr
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectSkinfr
dc.subjectContractionfr
dc.subjectKeratinocytefr
dc.subjectAir–liquid maturationfr
dc.subjectDifferentiationfr
dc.subjectFibroblastfr
dc.subject.rvmPeau artificiellefr
dc.subject.rvmContractilitéfr
dc.subject.rvmTissus (Histologie) -- Culturefr
dc.titleMinimal contraction for tissue-engineered skin substitutes when matured at the air–liquid interfacefr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationJournal of Tissue Engineering and Regenerative Medicine, Vol. 7 (6), 452–460 (2013)fr
dspace.accessstatus.time2023-03-26 18:04:06
dspace.entity.typePublication
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rioxxterms.project.funder-nameCanadian Institutes of Health Researchfr
rioxxterms.versionVoRfr
rioxxterms.version-of-recordhttps://doi.org/10.1002/term.543fr
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