Publication :
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis

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Date
2022-05-25
Auteurs
Kuo, Alan
Chovatia, Mansi
Johnson, Jenifer
Lipzen, Anna
Barry, Kerrie W.
Grigoriev, Igor V.
Direction de publication
Direction de recherche
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Éditeur
Springer Nature
Projets de recherche
Structures organisationnelles
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Résumé
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na+-H+ antiporters and Na+ -Pi symporters as salinity decreases, but the K+ channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation.
Description
Revue
Communications Biology, Vol. 5 (500) (2022)
DOI
10.1038/s42003-022-03461-2
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Type de document
article de recherche