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Characterization of a new virulent phage (MLC-A) of Lactobacillus paracasei

bul.description.provenanceeb bde spbfr
bul.rights.dateAccepPubl2006-02-20fr
bul.rights.periodeEmbargoforeverfr
bul.rights.raisonEmbargoInfiniPour que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de Journal of Dairy Science, il faudrait déposer la version acceptée pour publication, incluant toutes les modifications demandées, mais sans la mise en page de la revue. Pour ce faire, effectuez une demande de modification à l’aide de la liste des dépôts diffusés à partir du tableau de suivi.fr
bul.rights.typeDatedateAcceptationfr
dc.contributor.authorCapra, María Luján
dc.contributor.authorMoineau, Sylvain
dc.contributor.authorLuján Quiberoni, Andrea del
dc.contributor.authorAckermann, Hans-W.
dc.contributor.authorReinheimer, Jorge Alberto
dc.coverage.spatialArgentinefr
dc.date.accessioned2020-04-01T14:57:27Z
dc.date.available9999-12-31
dc.date.issued2010-03-03fr
dc.description.abstractA new virulent bacteriophage (MLC-A) was recently isolated in Argentina from a probiotic dairy product containing a strain of Lactobacillus paracasei. Observation of the lysate with an electron microscope revealed bacteriophage particles with an icosahedral capsid of 57 ± 2 nm; with a collar and a noncontractile tail of 156 ± 3 nm terminating with a baseplate to which a tail fiber was attached. Therefore, phage MLC-A belongs to the Siphoviridae family. This phage was able to survive the pasteurization process and was resistant to alcohols and sodium hypochlorite (400 mg/kg). Only peracetic acid could inactivate high-titer suspensions of phages in a short time. The maximum rates of phage adsorption to its host cells were obtained at 30°C with a pH between 5 and 7, and in the presence of calcium or magnesium ions. The host range of phage MLC-A encompassed L. paracasei and Lactobacillus casei strains, but it was not able to infect Lactobacillus rhamnosus or Lactobacillus gasseri strains. One-step growth kinetics of its lytic development revealed latent and burst periods of 30 and 135 min, respectively, with a burst size of about 69 ± 4 plaque-forming units per infected cell. Phage MLC-A had a distinctive restriction profile when compared with the 2 well-studied Lactobacillus phages, PL-1 and J-1. The genome size of the MLC-A phage was estimated to be approximately 37 kb. This study presents the description of the first phage specific for L. paracasei isolated in Argentina. The isolation of phage MLC-A indicates that, beside lactic acid bacteria starters, probiotic cultures can also be sensitive to virulent phages in industrial processes.fr
dc.identifier.doi10.3168/jds.S0022-0302(06)72314-1fr
dc.identifier.issn0022-0302fr
dc.identifier.pubmed16772557fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/38533
dc.languageengfr
dc.publisherAmerican Dairy Science Associationfr
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectLactobacillus caseifr
dc.subjectBacteriophagefr
dc.subjectFermented milkfr
dc.subject.rvmLactobacillus caseifr
dc.subject.rvmBactériophagesfr
dc.subject.rvmLait fermentéfr
dc.titleCharacterization of a new virulent phage (MLC-A) of Lactobacillus paracaseifr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationJournal of Dairy Science, Vol. 89 (7), 2414–2423 (2006)fr
dcterms.date.accepted2006-02-20
dspace.accessstatus.time2023-01-26 18:01:42
dspace.entity.typePublication
relation.isAuthorOfPublication599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isAuthorOfPublicationd1ec6901-44a9-45a6-bacd-b3dd9452e3fe
relation.isAuthorOfPublication.latestForDiscovery599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.projectPIP 02035/2000-2002fr
rioxxterms.project.funder-nameConsejo Nacional de Investigaciones Científicas y Técnicasfr
rioxxterms.versionVoRfr
rioxxterms.version-of-recordhttps://doi.org/10.3168/jds.S0022-0302(06)72314-1fr
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