Publication :
Skin equivalent produced with human collagen

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Date
1995-06-01
Direction de publication
Direction de recherche
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Éditeur
Society for In Vitro Biology
Projets de recherche
Structures organisationnelles
Numéro de revue
Résumé
Several studies have recently been conducted on cultured skin equivalent (SE), prepared using human keratinocytes seeded on various types of dermal equivalents (DE). We previously showed the advantages of our anchorage method in preventing the severe surface reduction of DE due to fibroblast contractile properties in vitro. A new anchored human SE was established in our laboratory in order to obtain a bioengineered tissue that would possess the appropriate histological and biological properties. In order to compare the effects of different collagen origins on the evolution of SE in vitro, human keratinocytes were seeded on three types of anchored DE. A comparative study was carried out between bovine SE (bSE), human SE (hSE), and human skin equivalent containing additional dermal matrix components (hSE +). Immunohistological analysis showed that hSE and hSE+ presented good structural organization, including the deposition of several basement membrane constituents. Higher amounts of transglutaminase, ceramides, and keratin 1 were detected in the epidermal layers of all SE when cultured at the air-liquid interface. However, a 92 kDa gelatinase activity was higher in bovine skin equivalent (bSE) compared to hSE cultures. The use of human collagens comparatively to bovine collagen as SE matricial component delayed the degradation of the dermal layer in culture.
Description
Revue
In Vitro Cellular and Developmental Biology - Animal, Vol. 31 (6), 432–439 (1995)
DOI
10.1007/BF02634255
URL vers la version publiée
Mots-clés
Skin substitute , Human collagen
Citation
Type de document
article de recherche