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Publication :
Optimization of preservation methods allows deeper insights into changes of raw milk microbiota

ali.license-refhttps://creativecommons.org/licenses/by/4.0fr
ali.license-ref.start-date2020-03-23fr
bul.description.provenanceec spbfr
bul.rights.addendumExemption des frais de publication en libre accès.fr
bul.rights.dateAccepPubl2020-03-05fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorRoy, Denis
dc.contributor.authorGroleau, Gisèle.
dc.contributor.authorDufour, Simon
dc.contributor.authorOuamba, Alexandre J. K.
dc.date.accessioned2020-03-27T15:20:36Z
dc.date.available2020-03-27T15:20:36Z
dc.date.issued2020-03-05
dc.description.abstractThe temporal instability of raw milk microbiota drastically affects the reliability of microbiome studies. However, little is known about the microbial integrity in preserved samples. Raw cow milk samples were preserved with azidiol or bronopol and stored at 4 °C, or with dimethyl sulfoxide (DMSO) or a mixture of azidiol and DMSO and stored at −20 °C for up to 30 days. Aliquots of 5-, 10-, and 30-day post-storage were treated with propidium monoazide (PMA), then analyzed by sequencing the 16S rRNA gene V3-V4 and V6-V8 regions. The V6-V8 gave a higher richness and lower diversity than the V3-V4 region. After 5-day storage at 4 °C, the microbiota of unpreserved samples was characterized by a drastic decrease in diversity, and a significant shift in community structure. The treatment with azidiol and DMSO conferred the best community stabilization in preserved raw milk. Interestingly, the azidiol treatment performed as well for up to 10 days, thus appearing as a suitable alternative. However, neither azidiol nor bronopol could minimize fungal proliferation as revealed by PMA-qPCR assays. This study demonstrates the preservative ability of a mixture of azidiol and DMSO and provides deeper insights into the microbial changes occurring during the cold storage of preserved raw milk.fr
dc.identifier.doi10.3390/microorganisms8030368fr
dc.identifier.issn2076-2607fr
dc.identifier.pubmed32151050fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/38408
dc.languageengfr
dc.publisherMDPI AGfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectRaw milk microbiotafr
dc.subjectPreservation methodfr
dc.subjectAzidiolfr
dc.subjectBronopolfr
dc.subjectDimethyl sulfoxidefr
dc.subject.rvmLait -- Microbiologiefr
dc.subject.rvmLait -- Conservationfr
dc.subject.rvmAliments -- Conservateursfr
dc.titleOptimization of preservation methods allows deeper insights into changes of raw milk microbiotafr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationMicroorganisms, Vol. 8 (3) (2020)fr
dspace.accessstatus.time2024-03-23 18:01:24
dspace.entity.typePublication
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relation.isAuthorOfPublication22fdaff5-345e-43e8-af34-f53d1ef92dda
relation.isAuthorOfPublication8b1d95e0-8264-4fb7-a68d-1d76e18d84e5
relation.isAuthorOfPublication.latestForDiscovery226104f7-e8be-4c0c-bbc8-7b8ebc259f03
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rioxxterms.project.funder-nameRegroupement de recherche pour un lait de qualité optimale (Op+lait)fr
rioxxterms.project.funder-nameFonds de Recherche du Québec - Nature et Technologiesfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.3390/microorganisms8030368fr

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