Publication :
Development of an encapsulation system for the protection and controlled release of antimicrobial nisin at meat cooking temperature

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Date
2013-04-24
Direction de publication
Direction de recherche
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ISSN de la revue
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Éditeur
Canadian Center of Science and Education
Projets de recherche
Structures organisationnelles
Numéro de revue
Résumé
Nisin is an antimicrobial peptide produced by Lactococcus lactis spp. lactis widely investigated for use in foods as a natural antimicrobial. However, its effective use in meat products is restricted notably by its reaction with meat constituents (including glutathione) in raw meat. The purpose of this study was to develop an encapsulation system that would optimize nisin activity when used in meat. To achieve this goal, an encapsulation in dipalmitoylphosphatidylcholine (DPPC) liposomes was developed. DPPC liposomes were formed in phosphate buffer with or without nisin. The encapsulation efficiency of nisin in liposomes was greater than 46 ± 2%. The median size of nisin-loaded liposomes was 495 nm, compared to 170 nm for empty liposomes. The liposomes containing nisin were stable for up to 7 days at 4°C but a zone of inhibition was observed afterwards. Stability of the liposome to heat was also tested and demonstrated that above 37°C nisin was released from the melted liposomes to form zones of inhibition. Activity of free and encapsulated nisin was tested in raw and cooked ground beef (71°C). Free nisin lost its activity in raw beef but DPPC-encapsulated nisin remained active and was released upon melting of the liposome during heat treatment.
Description
Revue
Journal of Food Research, Vol. 2 (3), 36-45 (2013)
DOI
10.5539/jfr.v2n3p36
URL vers la version publiée
Mots-clés
Antimicrobial , Delivery system , Encapsulation , Liposome , Meat , Nisin
Citation
Type de document
article de recherche