Publication :
Direct detection of lactococcal bacteriophages in cheese whey using DNA probes

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Date
1992-04-01
Direction de publication
Direction de recherche
Titre de la revue
ISSN de la revue
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Éditeur
ScienceDirect
Projets de recherche
Structures organisationnelles
Numéro de revue
Résumé
We have designed a new method for the rapid detection of lactococcal phage directly in milk samples. The method was based on a dot blot analysis and did not require a biological assay with sensitive indicator strains. Culture media or whey permeate samples containing phage were spotted directly onto a nylon membrane and the phage were lysed in situ prior to hybridization. For skim milk, whole milk and whey samples, the samples were first treated with 50 mM EDTA, 20% Triton X-100 and heated at 60°C for 5 min, prior to spotting on the membrane. The detection limit was approximately between 105 and 107 pfu/spot. A large number of samples could be processed simultaneously and the results were obtained within 24 h.
Description
Revue
FEMS Microbiology Letters, Vol. 92 (2), 169–174 (1992)
DOI
10.1111/j.1574-6968.1992.tb05254.x
URL vers la version publiée
Mots-clés
Lactococcus , Phage , Probe , Cheese , Whey
Citation
Type de document
article de recherche