This study aims to evaluate the potential of milkweed as potential candidate to construct biodegradable scaffold for bone regeneration. A mat made of milkweed, polyethylene, and polypropylene was treated with an atmospheric pressure plasma to functionalize the surface of the polymer assembly with carboxylic acid groups, which enable to conjugate bioactive molecules, while accelerating the degradation of milkweed. Degradation tests demonstrated substantial decrease of the weight of the treated polymer mat as compared to untreated one. Biological assays revealed that the polymer assembly promoted preosteoblast MC3T3 cells recruitment with a significant enhancement observed on the RGD-grafted polymer mat.