Publication :
Solution and electron microscopy characterization of lactococcal phage baseplates expressed in Escherichia coli

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Campanacci, Valérie
Veesler, David
Lichière, Julie
Blangy, Stéphanie
Sciara, Giuliano
Sinderen, Douwe van
Bron, Patrick
Cambillau, Christian
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Direction de recherche
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Academic Press
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Structures organisationnelles
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We report here the characterization of several large structural protein complexes forming the baseplates (or part of them) of Siphoviridae phages infecting Lactococcus lactis: TP901-1, Tuc2009 and p2. We revisited a "block cloning" expression strategy and extended this approach to genomic fragments encoding proteins whose interacting partners have not yet been clearly identified. Biophysical characterization of some of these complexes using circular dichroism and size exclusion chromatography, coupled with on-line light scattering and refractometry, demonstrated that the over-produced recombinant proteins interact with each other to form large (up to 1.9MDa) and stable baseplate assemblies. Some of these complexes were characterized by electron microscopy confirming their structural homogeneity as well as providing a picture of their overall molecular shapes and symmetry. Finally, using these results, we were able to highlight similarities and differences with the well characterized much larger baseplate of the myophage T4.

Journal of structural biology, Vol. 172 (1), 75–84 (2010)
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Operon expression , Lactococcus lactis phage , Multi-protein complex , Multi-angle light scattering , Receptor binding protein , Baseplate , Electron microscopy
Type de document
article de recherche