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Temporal changes in gene expression profile during mature adipocyte dedifferentiation

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bul.description.provenanceeb autorité-manquante : Lapointe - spbfr
bul.rights.addendumDroits de diffusion transférés à l'éditeur.fr
bul.rights.dateAccepPubl2017-03-19fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorGuénard, Frédéric
dc.contributor.authorBiron, Simon
dc.contributor.authorLapointe, Marc
dc.contributor.authorVohl, Marie-Claude
dc.contributor.authorCôté, Julie Anne
dc.contributor.authorLessard, Julie.
dc.contributor.authorTchernof, André
dc.date.accessioned2020-08-26T17:42:46Z
dc.date.available2020-08-26T17:42:46Z
dc.date.issued2017-03-19
dc.description.abstractObjective. To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture. Methods. Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI. Isolated adipocytes were dedifferentiated in ceiling culture. Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays. Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions. Results. Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12. Most of these genes coded for proteins involved in adipocyte functions (LIPE, PLIN1, DGAT2, PNPLA2, ADIPOQ, CEBPA, LPL, FABP4, SCD, INSR, and LEP). Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 (WNT5A, KITLG, and FGF5). Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (COL1A1, COL1A2, and COL6A3, MMP1, and TGFB1). Conclusion. Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellular development, and cell cycle.fr
dc.identifier.doi10.1155/2017/5149362fr
dc.identifier.issn2314-436Xfr
dc.identifier.pubmed28409151fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/40214
dc.languageengfr
dc.publisherHindawi Publishing Corporationfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectAdipocytefr
dc.subjectDedifferentiationfr
dc.subjectTranscriptomicfr
dc.subjectMolecular pathwaysfr
dc.subject.rvmAdipocytesfr
dc.subject.rvmCellules -- Différenciationfr
dc.subject.rvmExpression géniquefr
dc.subject.rvmTranscriptomiquefr
dc.titleTemporal changes in gene expression profile during mature adipocyte dedifferentiationfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationInternational Journal of Genomicsfr
dspace.accessstatus.time2023-01-26 18:00:29
dspace.entity.typePublication
relation.isAuthorOfPublication118048f6-7f60-4c91-afff-eb6cf5e11ca4
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relation.isAuthorOfPublication.latestForDiscovery118048f6-7f60-4c91-afff-eb6cf5e11ca4
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rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.versionAMfr
rioxxterms.version-of-recordhttps://doi.org/10.1155/2017/5149362fr
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