Publication :
Investigating Lactococcus lactis MG1363 response to phage p2 infection at the proteome level

bul.description.provenanceeb bde spbfr
bul.rights.dateAccepPubl2019-01-24fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorLemay, Marie-Laurence
dc.contributor.authorOtto, Andreas Willy
dc.contributor.authorMoineau, Sylvain
dc.contributor.authorMaaß, Sandra
dc.contributor.authorPlate, Kristina
dc.contributor.authorBecher, Dörte
dc.date.accessioned2020-03-31T17:48:08Z
dc.date.available2020-03-31T17:48:08Z
dc.date.issued2019-01-24
dc.description.abstractPhages are viruses that specifically infect and eventually kill their bacterial hosts. Bacterial fermentation and biotechnology industries see them as enemies, however, they are also investigated as antibacterial agents for the treatment or prevention of bacterial infections in various sectors. They also play key ecological roles in all ecosystems. Despite decades of research some aspects of phage biology are still poorly understood. In this study, we used label-free quantitative proteomics to reveal the proteotypes of Lactococcus lactis MG1363 during infection by the virulent phage p2, a model for studying the biology of phages infecting Gram-positive bacteria. Our approach resulted in the high-confidence detection and quantification of 59% of the theoretical bacterial proteome, including 226 bacterial proteins detected only during phage infection and 6 proteins unique to uninfected bacteria. We also identified many bacterial proteins of differing abundance during the infection. Using this high-throughput proteomic datasets, we selected specific bacterial genes for inactivation using CRISPR-Cas9 to investigate their involvement in phage replication. One knockout mutant lacking gene llmg_0219 showed resistance to phage p2 because of a deficiency in phage adsorption. Furthermore, we detected and quantified 78% of the theoretical phage proteome and identified many proteins of phage p2 that had not been previously detected. Among others, we uncovered a conserved small phage protein (pORFN1) coded by an unannotated gene. We also applied a targeted approach to achieve greater sensitivity and identify undetected phage proteins that were expected to be present. This allowed us to follow the fate of pORF46, a small phage protein of low abundance. In summary, this work offers a unique view of the virulent phages' takeover of bacterial cells and provides novel information on phage-host interactions.fr
dc.identifier.doi10.1074/mcp.RA118.001135fr
dc.identifier.issn1535-9476fr
dc.identifier.pubmed30679258fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/38512
dc.languageengfr
dc.publisherAmerican Society for Biochemistry and Molecular Biologyfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectMass Spectrometryfr
dc.subjectLabel-free quantificationfr
dc.subjectBacteriafr
dc.subjectVirusesfr
dc.subjectProteogenomicsfr
dc.subjectPhage infectionfr
dc.subjectLactococcus lactisfr
dc.subjectGenome editingfr
dc.subject.rvmLactococcus lactisfr
dc.subject.rvmBactériophage P2fr
dc.subject.rvmProtéomesfr
dc.subject.rvmRelations hôte-virusfr
dc.titleInvestigating Lactococcus lactis MG1363 response to phage p2 infection at the proteome levelfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationMolecular and cellular proteomics, MCP, Vol. 18 (4), 704-714 (2019)fr
dspace.accessstatus.time2023-03-26 18:00:41
dspace.entity.typePublication
relation.isAuthorOfPublication579b25df-c113-4616-8b8c-44bb3c0a9d12
relation.isAuthorOfPublication599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isAuthorOfPublication.latestForDiscovery579b25df-c113-4616-8b8c-44bb3c0a9d12
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameFonds de Recherche du Québec - Nature et Technologiesfr
rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.versionAMfr
rioxxterms.version-of-recordhttps://doi.org/10.1074/mcp.RA118.001135fr
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