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Regulation of gap-junctional communication between cumulus cells during in vitro maturation in swine, a gap-FRAP study

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bul.description.provenancemcp spbfr
bul.rights.dateAccepPubl2012-05-30fr
bul.rights.periodeEmbargoforeverfr
bul.rights.raisonEmbargoInfiniPour que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de Biology of Reproduction, il faudrait déposer la version acceptée pour publication, incluant toutes les modifications demandées, mais sans la mise en page de la revue. Pour ce faire, effectuez une demande de modification à l’aide de la liste des dépôts diffusés à partir du tableau de suivi.fr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorSantiquet, Nicolas
dc.contributor.authorRichard, François J.
dc.contributor.authorDevelle, Yann
dc.contributor.authorRobert, Claude
dc.contributor.authorLaroche, Anthony
dc.date.accessioned2019-06-04T19:35:30Z
dc.date.available9999-12-31
dc.date.issued2012-05-30
dc.description.abstractIntercellular gap-junctional communication (GJC) plays an important role in ovarian cell physiology. Closure of GJC has been proposed to be involved in oocyte maturation, particularly in the resumption of meiosis, both in vivo and in vitro, by controlling the flow of meiosis inhibitors, such as cAMP and cGMP. Understanding how GJC dynamics are regulated during in vitro maturation (IVM) could provide a powerful tool for controlling meiotic resumption and oocyte maturation in vitro. Since little is known about the GJC dynamic regulation between cumulus cells, we have developed an assay based on recovery of calcein fluorescence in photobleached cumulus cells, a gap-FRAP assay. The GJC profile has been characterized during the first hours of porcine IVM. We showed that equine chorionic gonadotropin (eCG) and epidermal growth factor (EGF) down-regulated GJC effectiveness between cumulus cells. However, human chorionic gonadotropin was not down-regulating GJC effectiveness. We also showed that the GJC network expanded during this period and that this effect was not regulated by gonadotropins. Porcine follicular fluid present in the maturation medium also had an impact on GJC regulation, increasing GJC network establishment and the effectiveness of calcein transfer rate between cumulus cells. These results show that both eCG and EGF are regulating the decrease in GJC effectiveness after 4.5 h of IVM, while the network extension is gonadotropin independent. Regulation of GJC between cumulus cells would then be specifically regulated during in vitro IVM.fr
dc.identifier.doi10.1095/biolreprod.112.099754fr
dc.identifier.issn0006-3363fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/35095
dc.languageengfr
dc.publisherSociety for the Study of Reproductionfr
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectcumulus cellsfr
dc.subjectgap junction communicationfr
dc.subjectgonadotropinsfr
dc.subjectin vitro maturationfr
dc.subjectporcinefr
dc.subjectpigfr
dc.subject.rvmCumulus (Anatomie)fr
dc.subject.rvmGonadotrophinesfr
dc.subject.rvmPorcs (Animaux de laboratoire)fr
dc.subject.rvmOvocytesfr
dc.titleRegulation of gap-junctional communication between cumulus cells during in vitro maturation in swine, a gap-FRAP studyfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationBiology of reproduction, Vol. 87 ( 2), 46, 1-8 (2012)fr
dspace.accessstatus.time2023-05-24 18:00:25
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery81b46573-4e66-4f3d-81cc-64347119e872
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rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttp://dx.doi.org/10.1095/biolreprod.112.099754fr
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