Publication :
The gametic synapse : RNA transfer to the bovine oocyte

Notice brève
bul.description.provenancelf spbfr
bul.rights.dateAccepPubl2014-10-01fr
bul.rights.periodeEmbargoforeverfr
bul.rights.raisonEmbargoInfiniPour que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de Biology of Reproduction, il faudrait déposer la version acceptée pour publication, incluant toutes les modifications demandées, mais sans la mise en page de la revue. Pour ce faire, effectuez une demande de modification à l’aide de la liste des dépôts diffusés à partir du tableau de suivi.fr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorGilbert, Isabelle
dc.contributor.authorCaballero, Julieta
dc.contributor.authorTossou, Prudencio
dc.contributor.authorKhandjian, Edward William
dc.contributor.authorMacaulay, Angus
dc.contributor.authorRichard, François J.
dc.contributor.authorBarreto, Rodrigo
dc.contributor.authorClarke, Hugh James
dc.contributor.authorRobert, Claude
dc.contributor.authorFournier, Éric
dc.contributor.authorSirard, Marc-André
dc.contributor.authorHyttel, P.
dc.date.accessioned2019-06-04T19:56:17Z
dc.date.available9999-12-31
dc.date.issued2014-10-01
dc.description.abstractEven after several decades of quiescent storage in the ovary, the female germ cell is capable of reinitiating transcription to build the reserves that are essential to support early embryonic development. In the current model of mammalian oogenesis, there exists bilateral communication between the gamete and the surrounding cells that is limited to paracrine signaling and direct transfer of small molecules via gap junctions existing at the end of the somatic cells' projections that are in contact with the oolemma. The purpose of this work was to explore the role of cumulus cell projections as a means of conductance of large molecules, including RNA, to the mammalian oocyte. By studying nascent RNA with confocal and transmission electron microscopy in combination with transcript detection, we show that the somatic cells surrounding the fully grown bovine oocyte contribute to the maternal reserves by actively transferring large cargo, including mRNA and long noncoding RNA. This occurrence was further demonstrated by the reconstruction of cumulus-oocyte complexes with transfected cumulus cells transferring a synthetic transcript. We propose selective transfer of transcripts occurs, the delivery of which is supported by a remarkable synapselike vesicular trafficking connection between the cumulus cells and the gamete. This unexpected exogenous contribution to the maternal stores offers a new perspective on the determinants of female fertility.fr
dc.identifier.doi10.1095/biolreprod.114.119867fr
dc.identifier.issn0006-3363fr
dc.identifier.pubmed25143353fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/35081
dc.languageengfr
dc.publisherOxford University Pressfr
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectIntercellular communicationfr
dc.subjectIn vitro maturation (IVM)fr
dc.subjectOocyte developmentfr
dc.subjectOocyte maturationfr
dc.subject.rvmTransduction du signal cellulairefr
dc.subject.rvmOvocytesfr
dc.subject.rvmARNfr
dc.subject.rvmCumulus (Anatomie)fr
dc.subject.rvmSynapsesfr
dc.titleThe gametic synapse : RNA transfer to the bovine oocytefr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationBiology of reproduction, Vol 91 (4), 90, 1-12 (2014)fr
dspace.accessstatus.time2023-06-08 18:00:20
dspace.entity.typePublication
relation.isAuthorOfPublication3fae371d-cf2c-414a-a985-76739ee4c839
relation.isAuthorOfPublication3fbcf3fc-1d7b-4a05-a9d3-c3479cfcdb63
relation.isAuthorOfPublication4b430063-d674-4a98-b063-b12ad540e839
relation.isAuthorOfPublication5ea4fa03-bab0-4eaf-801f-9f2b30b1e236
relation.isAuthorOfPublication7fe52513-1089-4cfe-b499-6c2bb4495b2f
relation.isAuthorOfPublication8367ef55-3028-4735-a279-764d526ea830
relation.isAuthorOfPublication89867557-a37c-4c4e-b70b-79b6bfd95030
relation.isAuthorOfPublicationa63d9225-66db-4614-bb90-b9fc31255a4e
relation.isAuthorOfPublicationa91d68ca-0c0e-4241-8d83-0294f66a76ea
relation.isAuthorOfPublicationebd0d20d-b7af-4f60-aeb4-203925d521fa
relation.isAuthorOfPublication.latestForDiscovery3fae371d-cf2c-414a-a985-76739ee4c839
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameFonds Québécois de la Recherche sur la Nature et les Technologiesfr
rioxxterms.project.funder-nameCanadian Institutes of Health Researchfr
rioxxterms.project.funder-nameInstitute of Human Development, Child and Youth Healthfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1095/biolreprod.114.119867fr
Fichiers
Bundle original
Voici les éléments 1 - 1 sur 1
Vignette d'image
Nom :
Macaulay et al. - 2014 - The Gametic Synapse RNA Transfer to the Bovine Oocyte1.pdf
Taille :
1.02 MB
Format :
Adobe Portable Document Format
Description :
Télécharger
Collections
Articles publiés dans des revues avec comité de lecture