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Personne :
Laflamme, Karina

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Laflamme

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Karina

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Département de biologie moléculaire, Faculté de médecine, Université Laval

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ncf11853544

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Voici les éléments 1 - 3 sur 3
  • PublicationRestreint
    Recent optimization of a tissue engineered blood vessel : the LOEX experience
    (Excerpta Medica Foundation, 2004-05-01) Grenier, Guillaume.; Germain, Lucie; Auger, François A.; Rémy-Zolghadri, Murielle; Laflamme, Karina
    Creating a blood vessel by tissue engineering is one of the most demanding goals in tissue engineering. Our laboratory developed, using the self-assembly approach, the first completely biological tissue engineered blood vessel (TEBV) constituted of living human cells in the absence of any synthetic or exogenous material. The phenotypic and functional variations of smooth muscle cell (SMC) are of paramount importance in TEBV reconstruction. Thus, the phenotype and extracellular matrix (ECM) production of SMC were studied along the whole sequence of TEBV production. The functional and mechanical properties can be greatly enhanced by active cell orientation in the ECM. Accordingly, the method of preparing living tissue engineered sheets was modified to obtain an optimal alignment of SMC before rolling them into a tubular form. These results have allowed us to create a better TEBV.
  • PublicationRestreint
    Tissue-engineered human vascular media produced in vitro by the self-assembly approach present functional properties similar to those of their native blood vessels
    (Mary Ann Liebert, Inc, 2006-09-12) Germain, Lucie; Pouliot, Stéphanie; Roberge, Charles; D’Orléans-Juste, Pedro; Auger, François A.; Laflamme, Karina
    We have developed a tissue-engineering approach for the production of a completely biological blood vessel from cultured human cells. In the present study, we took advantage of this tissue-engineering method to demonstrate that it can be used to reproduce the subtle differences in the expression of receptors present on the media of native human blood vessels. Indeed, a small percentage (3 of 18) of native human umbilical cord veins (HUCVs) responded to endothelin, the most powerful vasopressor agent known to date, via both endothelin A (ETA) and endothelin B (ETB) receptor activation. In contrast, most HUCVs tested responded toETviaETA receptor activation only. Tissue-engineered vascular media (TEVM) were next reconstructed by using vascular smooth muscle cells (VSMCs) isolated and cultured from HUCVs expressing both ETA and ETB receptors to determine the functional integrity of our TEVM model. The reconstructed TEVM presents an endothelin response similar to that of respective HUCVs from which VSMCs were isolated. Reverse transcriptase polymerase chain reaction on TEVM reconstructed in vitro correlated these vasocontractile profiles by showing the presence of messenger RNA for both ETA and ETB receptors. Taken together with recently published results on TEVM expressing only ETA receptor, these results show that our reconstructed TEVM present a similar ET response profile as the blood vessel from which the VSMCs were isolated and cultured. These findings indicate that subtle differences, such as receptor expression, are preserved in the reconstructed tissue. Therefore, our TEVM offers a valuable human in vitro model with which to study the functionality of human blood vessels, such as their vasoactive response, or to perform pharmacologic studies.
  • PublicationRestreint
    Tissue-engineered human vascular media with a functional endothelin system.
    (American Heart Association etc, 2005-02-01) Labonté, Julie; Germain, Lucie; Pouliot, Stéphanie; Roberge, Charles; D’Orléans-Juste, Pedro; Auger, François A.; Laflamme, Karina
    Background— Cardiovascular diseases remain a major cause of death and disability in the Western world. Among the various approaches adopted to counteract the morbidity associated with these diseases, surgical procedures and cardiac and vascular xenotransplantations or allotransplantations are routinely performed. The suitable vascular graft would be as close as possible to the native and healthy vessel composed exclusively of human components provided by the patient and would adapt to the donor’s hemodynamics. We have developed such a tissue-engineered human blood vessel reconstructed with human cells. Because endothelin is the most potent vasopressor known to date, we were interested in investigating the functionality of the endothelinergic system in our reconstructed human blood vessel. Methods and Results— Vasoconstriction studies were performed with nonselective and selective agonists and antagonists to demonstrate that ETA receptors were present and functional in tissue-engineered human vascular media constructed with the self-assembly method. Reverse-transcriptase polymerase chain reaction studies demonstrated that mRNA of the ETA but not the ETB receptor was present in these human tissue–engineered blood vessels. Furthermore, we demonstrated that the endothelin-converting enzyme, the main enzyme responsible for the formation of the biologically active endothelin peptides, was present and functional in these same bioengineered vascular media. Conclusions— Our results suggest that the media component of our tissue-engineered blood vessel has the potential of controlling vascular resistance via the presence of functional endothelin ETA receptors and endothelin-converting enzyme. endothelinmuscle contractionmyocytes, smooth m