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Lemieux, Simone

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Lemieux

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Simone

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Université Laval. École de nutrition

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ncf10500689

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Voici les éléments 1 - 10 sur 98
  • PublicationAccès libre
    Prevention of potential adverse metabolic effects of a supplementation with omega-3 fatty acids using a genetic score approach
    (Karger, 2019-11-29) Franck, Maximilien; Guénard, Frédéric; Toro Martin, Juan de; Rudkowska, Iwona; Lamarche, Benoît; Lemieux, Simone; Vohl, Marie-Claude; Couture, Patrick
    Introduction: The consumption of long-chain omega-3 polyunsaturated fatty acids (n-3 PUFA) has been reported to have beneficial health effects, notably, by reducing plasma triglyceride levels. Nonetheless, a concomitant decrease in insulin sensitivity has also been observed, but is highly variable among subjects. Herein, we aimed to determine the importance of the genetic background in the interindividual variability of the insulin sensitivity response following an n-3 PUFA supplementation. Methods: A total of 210 participants completed a 6-week n-3 PUFA supplementation with 5 g/day of fish oil (providing 1.9–2.2 g of eicosapentaenoic acid + 1.1 g of docosahexaenoic acid). Insulin resistance was estimated by the homeostatic model assessment (HOMA-IR), and participants were further classified as high-risk or low-risk depending on their HOMA-IR change following the n-3 PUFA supplementation, as compared to pre-supplementation values. Genome-wide genotyping data were obtained for 138 participants using HumanOmni-5-Quad BeadChips containing 4,301,331 single nucleotide polymorphisms. A genome-wide association analysis (GWAS) was carried out between high-risk and low-risk participants. The population study was split into training (60%) and testing (40%) datasets to assess the predictive accuracy of a genetic risk score (GRS) constructed by summing the number of risk alleles. Results: Following the n-3 PUFA supplementation, 32 participants had increased HOMA-IR as compared to initial values and were classified as high risk (23.2%), whereas remaining subjects were classified as low risk (n = 106, 76.8%). A total of 8 loci had frequency differences between high-risk and low-risk participants at a suggestive GWAS association threshold (p value <1 × 10–5). After applying 10-fold cross validation, the GRS showed a significant association with the risk of increased HOMA-IR in the testing dataset (OR = 3.16 [95% CI, 1.85–7.14]), with a predictive accuracy of 0.85, and explained 40% of variation in HOMA-IR change. Conclusions: These results suggest that the genetic background has a relevant role in the interindividual variability observed in the insulin sensitivity response following an n-3 PUFA supplementation. Subjects being at risk of insulin sensitivity lowering following an n-3 PUFA supplementation may be identified using genetic-based precision nutrition approaches
  • PublicationAccès libre
    Genome-wide association study of dietary pattern scores
    (MDPI, 2017-06-23) Guénard, Frédéric; Bouchard-Mercier, Annie; Rudkowska, Iwona; Lemieux, Simone; Vohl, Marie-Claude; Couture, Patrick
    Dietary patterns, representing global food supplies rather than specific nutrients or food intakes, have been associated with cardiovascular disease (CVD) incidence and mortality. The contribution of genetic factors in the determination of food intakes, preferences and dietary patterns has been previously established. The current study aimed to identify novel genetic factors associated with reported dietary pattern scores. Reported dietary patterns scores were derived from reported dietary intakes for the preceding month and were obtained through a food frequency questionnaire and genome-wide association study (GWAS) conducted in a study sample of 141 individuals. Reported Prudent and Western dietary patterns demonstrated nominal associations (p < 1 × 10−5) with 78 and 27 single nucleotide polymorphisms (SNPs), respectively. Among these, SNPs annotated to genes previously associated with neurological disorders, CVD risk factors and obesity were identified. Further assessment of SNPs demonstrated an impact on gene expression levels in blood for SNPs located within/near BCKDHB (p = 0.02) and the hypothalamic glucosensor PFKFB3 (p = 0.0004) genes, potentially mediated through an impact on the binding of transcription factors (TFs). Overrepresentations of glucose/energy homeostasis and hormone response TFs were also observed from SNP-surrounding sequences. Results from the current GWAS study suggest an interplay of genes involved in the metabolic response to dietary patterns on obesity, glucose metabolism and food-induced response in the brain in the adoption of dietary patterns.
  • PublicationRestreint
    Postmenopausal women with abdominal obesity choosing a nutritional approach for weight loss: A decisional needs assessment
    (Elsevier, 2016-08-19) Lapointe, Annie; Poirier, Nadine; Stacey, Dawn; Lemieux, Simone; Desroches, Sophie; Bégin, Catherine; Légaré, France
    Objectives: To identify the decisional needs of postmenopausal women with abdominal obesity choosing between two nutritional approaches for weight loss: a low-fat diet or a diet rich in fruit and vegetables. Study design: Our descriptive qualitative study was based on the Ottawa Decision Support Framework. Main outcome measures: Four focus groups were conducted with postmenopausal women. A thematic content analysis was performed to determine the decisional needs influencing the choice of a low-fat diet or a diet rich in fruit and vegetables. Results: Seventeen postmenopausal women participated in the study (median age 59 years). Most frequently reported decisional needs for each nutritional approach were sufficient levels of nutritional skills and knowledge, consideration of the physiological impacts and the sensory aspect of approaches, food availability, social support, finances and motivation. Partners, friends and daughters were considered as the most important individuals involved in the decision. Conclusions: We identified several decisional needs influencing postmenopausal women when choosing between a low-fat diet and a diet rich in fruit and vegetables. These findings could inform the design of decision support interventions that address the decisional needs of women for making and implementing informed decisions about a nutritional approach for weight loss.
  • PublicationRestreint
    Transcriptomic and metabolomic signatures of an n-3 polyunsaturated fatty acids 2 supplementation in a normolipidemic/normocholesterolemic Caucasian population
    (Elsevier, 2012-06-28) Thifault, Elisabeth; Paradis, Ann-Marie; Rudkowska, Iwona; Barbier, Olivier; Lemieux, Simone; Julien, Pierre; Vohl, Marie-Claude; Couture, Patrick; Tchernof, André
    OMIC technologies, including transcriptomics and metabolomics, may provide powerful tools for identifying the effects of nutrients on molecular functions and metabolic pathways. The objective was to investigate molecular and metabolic changes following n-3 polyunsaturated fatty acid (PUFA) supplementation in healthy subjects via traditional biomarkers as well as transcriptome and metabolome analyses. Thirteen men and 17 women followed a 2-week run-in period based on Canada's Food Guide and then underwent 6-week supplementation with n-3 PUFA (3 g/day). Traditional biochemical markers such as plasma lipids, inflammatory markers, glycemic parameters and erythrocyte fatty acid concentrations were measured. Changes in gene expression of peripheral blood mononuclear cells were assessed by microarrays, and metabolome profiles were assessed by mass spectrometry assay kit. After supplementation, plasma triglycerides decreased and erythrocyte n-3 PUFA concentrations increased to a similar extent in both genders. Further, plasma high-density lipoprotein cholesterol concentrations and fasting glucose levels increased in women after n-3 PUFA supplementation. N-3 PUFA supplementation changed the expression of 610 genes in men, whereas the expression of 250 genes was altered in women. Pathway analyses indicate changes in gene expression of the nuclear receptor peroxisome proliferator-activated receptor-alpha, nuclear transcription-factor kappaB, oxidative stress and activation of the oxidative stress response mediated by nuclear factor (erythroid-derived 2)-like 2. After n-3 PUFA supplementation, metabolomics profiles demonstrate an increase in acylcarnitines, hexose and leucine in men only and a decrease in saturation of glycerophosphatidylcholine and lysophosphatidylcholine concentrations in all subjects. Overall, traditional and novel biomarkers suggest that n-3 PUFA supplementation exerts cardioprotective effects.
  • PublicationAccès libre
    Associations between dietary protein sources, plasma BCAA and short-chain acylcarnitine levels in adults
    (M D P I AG, 2019-01-15) Guénard, Frédéric; Garneau, Véronique; Allam-Ndoul, Bénédicte; Pérusse, Louis; Lemieux, Simone; Vohl, Marie-Claude; Rousseau, Michèle
    Elevated plasma branched-chain amino acids (BCAA) and C3 and C5 acylcarnitines (AC) levels observed in individuals with insulin resistance (IR) might be influenced by dietary protein intakes. This study explores the associations between dietary protein sources, plasma BCAA levels and C3 and C5 ACs in normal weight (NW) or overweight (OW) individuals with or without metabolic syndrome (MS). Data from 199 men and women aged 18–55 years with complete metabolite profile were analyzed. Associations between metabolic parameters, protein sources, plasma BCAA and AC levels were tested. OW/MS+ consumed significantly more animal protein (p = 0.0388) and had higher plasma BCAA levels (p < 0.0001) than OW/MS− or NW/MS− individuals. Plasma BCAA levels were not associated with BCAA intakes in the whole cohort, while there was a trend for an association between plasma BCAA levels and red meat or with animal protein in OW/MS+. These associations were of weak magnitude. In NW/MS− individuals, the protein sources associated with BCAA levels varied greatly with adjustment for confounders. Plasma C3 and C5 ACs were associated with plasma BCAA levels in the whole cohort (p < 0.0001) and in subgroups based on OW and MS status. These results suggest a modest association of meat or animal protein intakes and an association of C3 and C5 ACs with plasma BCAA levels, obesity and MS.
  • PublicationAccès libre
    Epigenetic changes in blood leukocytes following an omega-3 fatty acid supplementation
    (BiomedCentral, 2017-04-26) Guénard, Frédéric; Rudkowska, Iwona; Lemieux, Simone; Tremblay, Bénédicte L.; Vohl, Marie-Claude; Couture, Patrick
    Background: Omega-3 polyunsaturated fatty acids (n-3 FAs) have several beneficial effects on cardiovascular (CV) disease risk factors. These effects on CV risk profile may be mediated by several factors, including epigenetic modifications. Our objective is to investigate, using genome-wide DNA methylation analyses, methylation changes following an n-3 FA supplementation in overweight and obese subjects and to identify specific biological pathways potentially altered by the supplementation. Results: Blood leukocytes genome-wide DNA methylation profiles of 36 overweight and obese subjects before and after a 6-week supplementation with 3 g of n-3 FAs were compared using GenomeStudio software. After supplementation, 308 CpG sites, assigned to 231 genes, were differentially methylated (FDR-corrected Diffscore ≥│13│~ P ≤ 0.05). Using Ingenuity Pathway Analysis system, a total of 55 pathways were significantly overrepresented following supplementation. Among these pathways, 16 were related to inflammatory and immune response, lipid metabolism, type 2 diabetes, and cardiovascular signaling. Changes in methylation levels of CpG sites within AKT3, ATF1, HDAC4, and IGFBP5 were correlated with changes in plasma triglyceride and glucose levels as well as with changes in the ratio of total cholesterol/HDL-cholesterol following the supplementation. Conclusions: These data provide key differences in blood leukocytes DNA methylation profiles of subjects following an n-3 FA supplementation, which brings new, potential insights on metabolic pathways underlying the effects of n-3 FAs on CV health.
  • PublicationAccès libre
    Relative validity of a web-based, self-administered, 24-hour dietary recall to evaluate adherence to Canadian dietary guidelines
    (Elsevier Science, 2018-05-24) Lafrenière, Jacynthe; Laramée, Catherine; Robitaille, Julie; Lamarche, Benoît; Lemieux, Simone
    Objectives: A new, web-based, 24-h recall (R24 W) was developed and programmed to assess adherence to Canadian dietary guidelines by automatically generating the Canadian Healthy Eating Index 2007 (C-HEI 2007). The aim of the present study was to determine the relative validity of the R24 W in terms of its ability to generate C-HEI 2007 scores that match those derived from a food record (FR). Methods: A total of 57 women and 50 men filled a 3-d FR and three 24-h food recalls with the R24 W. C-HEI 2007 was calculated with both dietary assessment tools and compared using de-attenuated correlations and kappa scores. Internal validity (correlations between total score and components) and external validity (comparison of the C-HEI 2007 by sex and age groups) were compared between the two dietary assessment methods. Results: The average C-HEI 2007 score obtained with the R24 W (59.4 ± 11.8) was significantly lower than the one obtained with the FR (62.6 ± 11.1). However, the de-attenuated correlation coefficient between C-HEI 2007 obtained either with R24 W or FR was 0.80 (P < 0.01) and 91.6% of participants were classified in the same or adjacent quartile of C-HEI 2007 with both tools. Women obtained a higher C-HEI 2007 mean score (P < 0.01) with both methods and older adults (age: >51 y) received a higher score than younger adults (age: 18-30 y; P < 0.01) with the R24 W but not with the FR (P = 0.22). Conclusions: Although the R24 W yields lower C-HEI 2007 scores compared with data from FR, the classification of individuals was highly consistent between the two measurements.
  • PublicationAccès libre
    Association between polymorphisms in phospholipase A2 genes and the plasma triglyceride response to an n-3 PUFA supplementation : a clinical trial
    (BioMed Central, 2015-02-21) Cormier, Hubert; Rudkowska, Iwona; Lemieux, Simone; Tremblay, Bénédicte L.; Vohl, Marie-Claude; Couture, Patrick
    Background: Fish oil-derived long-chain omega-3 (n-3) polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), reduce plasma triglyceride (TG) levels. Genetic factors such as single-nucleotide polymorphisms (SNPs) found in genes involved in metabolic pathways of n-3 PUFA could be responsible for well-recognized heterogeneity in plasma TG response to n-3 PUFA supplementation. Previous studies have shown that genes in the glycerophospholipid metabolism such as phospholipase A2 (PLA2) group II, IV, and VI, demonstrate changes in their expression levels in peripheral blood mononuclear cells (PBMCs) after n-3 PUFA supplementation. Methods: A total of 208 subjects consumed 3 g/day of n-3 PUFA for 6 weeks. Plasma lipids were measured before and after the supplementation period. Five SNPs in PLA2G2A, six in PLA2G2C, eight in PLA2G2D, six in PLA2G2F, 22 in PLA2G4A, five in PLA2G6, and nine in PLA2G7 were genotyped. The MIXED Procedure for repeated measures adjusted for age, sex, BMI, and energy intake was used in order to test whether the genotype, supplementation or interaction (genotype by supplementation) were associated with plasma TG levels. Results: The n-3 PUFA supplementation had an independent effect on plasma TG levels. Genotype effects on plasma TG levels were observed for rs2301475 in PLA2G2C, rs818571 in PLA2G2F, and rs1569480 in PLA2G4A. Genotype x supplementation interaction effects on plasma TG levels were observed for rs1805018 in PLA2G7 as well as for rs10752979, rs10737277, rs7540602, and rs3820185 in PLA2G4A. Conclusion: These results suggest that, SNPs in PLA2 genes may influence plasma TG levels during a supplementation with n-3 PUFA. This trial was registered at clinicaltrials.gov as NCT01343342.
  • PublicationRestreint
    GAD2 gene sequence variations are associated with eating behaviors and weight gain in women from the Quebec family study
    (Pergamon Press, 2009-08-15) Drapeau, Vicky; Choquette, Anne.; Bouchard, Claude; Pérusse, Louis; Tremblay, Angelo; Lemieux, Simone; Vohl, Marie-Claude
    The glutamate decarboxylase 2 (GAD2) gene encodes for the glutamic acid decarboxylase enzyme (GAD65), which is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. The objective of the present study was to test for association between GAD2 single-nucleotide polymorphisms (SNPs) and eating behaviors, dietary intake and obesity in subjects (n=873) from the Quebec Family Study (QFS). Energy and macronutrient intakes were measured using a 3-day dietary record and eating behaviors were assessed using the Three-Factor Eating Questionnaire (TFEQ). Six SNPs capturing about 90% of GAD2 gene variability were genotyped and tested for association with age- and BMI- adjusted phenotypes. No evidence of association was found in men. In women, a SNP (rs992990; c.61450 C>A) was associated with disinhibition (p=0.028), emotional susceptibility to disinhibition (p=0.0005) and susceptibility to hunger (p=0.028). Another SNP (rs7908975; c.8473A>C) was associated with carbohydrate (p=0.021) and lipid (p=0.021) intakes, disinhibition (p=0.011) and two of its subscales (emotional and situational susceptibility) as well as with avoidance of fattening foods (p=0.036). Six-year weight gain was two times higher in women carrying the variants associated with eating behaviors: 4.2kg (vs 2.1kg in non-carriers) in A-allele carriers of c.61450 C>A (p=0.038) and 4.9kg (vs 2.5kg in non-carriers) in C-allele carriers of c. 8473 A>C (p=0.013). The results suggest a role for the GAD2 gene in determining food intake, eating behaviors and weight gain over time in women.
  • PublicationAccès libre
    Eating behaviors of non-obese individuals with and without familial history of obesity
    (Cambridge University Press, 2008-09-10) Paradis, Ann-Marie; Pérusse, Louis; Godin, Gaston; Lemieux, Simone; Vohl, Marie-Claude
    The aim of the present study was to examine whether eating behaviours and their subscales are associated with familial history of obesity (FHO) in a cohort of 326 non-obese men and women. Anthropometric measurements, eating behaviours (Three-Factor Eating Questionnaire) and dietary intakes (FFQ) have been determined in a sample of 197 women and 129 men. A positive FHO (FHO+) was defined as having at least one obese first-degree relative and a negative FHO (FHO-) as no obese first-degree relative. Men with FHO+ had higher scores of cognitive dietary restraint and flexible restraint than men with FHO-. In women, those with FHO+ had a higher score of disinhibition than women with FHO-. In both men and women, eating behaviours were not significantly associated with the number of obese family members. However, having an obese mother was associated with higher scores of cognitive dietary restraint, flexible restraint and rigid restraint in women. These findings demonstrate that eating behaviours of non-obese subjects are different according to the presence or absence of obese family members. More specifically, having an obese mother is associated with a higher dietary restraint score in women.