Personne :
Labrie, Fernand

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Labrie
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Fernand
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Département de médecine moléculaire, Faculté de médecine, Université Laval
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  • Publication
    Restreint
    Detection of a novel mutation (stop 468) in exon 10 of the low-density lipoprotein receptor gene causing heterozygous familial hypercholesterolemia among French Canadians
    (Oxford University Press, 1994-09-01) Simard, Jacques; Moorjani, Sital; Labrie, Fernand; Lupien, Paul-J.; Vohl, Marie-Claude; Torres, Ana Lucia; Després, Jean-Pierre; Gagné, Claude; Couture, Patrick
  • Publication
    Accès libre
    Reproducibility, bioinformatic analysis and power of the SAGE method to evaluate changes in transcriptome
    (Information Retrieval Limited, 2005-02-01) Dinel, Stéphanie; Calvo, Ezequiel Luis; Bolduc, Carl; Belleau, Pascal; Piedboeuf, Bruno; Boivin, André; St-Amand, Jonny; Yoshioka, Mayumi; Labrie, Fernand; Snyder, Eric E.
    The serial analysis of gene expression (SAGE) method is used to study global gene expression in cells or tissues in various experimental conditions. However, its reproducibility has not yet been definitively assessed. In this study, we have evaluated the reproducibility of the SAGE method and identified the factors that affect it. The determination coefficient (R2) for the reproducibility of SAGE is 0.96. However, there are some factors that can affect the reproducibility of SAGE, such as the replication of concatemers and ditags, the number of sequenced tags and double PCR amplification of ditags. Thus, corrections for these factors must be made to ensure the reproducibility and accuracy of SAGE results. A bioinformatic analysis of SAGE data is also presented in order to eliminate these artifacts. Finally, the current study shows that increasing the number of sequenced tags improves the power of the method to detect transcripts and their regulation by experimental conditions.
  • Publication
    Accès libre
    Increased body fat mass explains the positive association between circulating estradiol and insulin resistance in postmenopausal women
    (American Physiological Society, 2017-05-08) Marchand, Geneviève B.; Carreau, Anne-Marie; Labrie, Fernand; Lemieux, Simone; Weisnagel, John; Tchernof, André; Bergeron, Jean
    The relationship between circulating estrogen levels and cardiometabolic risk factors such as insulin resistance is unclear in postmenopausal women. High estradiol (E2) levels have been reported to predict increased risk of type 2 diabetes in this population. We aimed to examine associations among estrogen levels, adiposity measurements, and cardiometabolic risk variables including insulin resistance in postmenopausal women. One hundred-one healthy participants (mean ± SD: age 57 ± 4 yr, BMI 27.9 ± 4.8 kg/m2) were included in the analysis. Fifteen plasma steroids or metabolites were measured by liquid chromatography-tandem mass spectrometry. Insulin sensitivity was assessed with a hyperinsulinemic-euglycemic clamp. Body composition and fat distribution were determined with hydrostatic weighing and computed tomography, respectively. Blood lipids and circulating cytokines were also measured. Circulating E2 was positively correlated with all adiposity indexes (r = 0.62 to 0.42, P < 0.0001) except waist-to-hip ratio. E2 was positively correlated with VLDL-cholesterol, plasma-, VLDL-, and HDL-triglyceride levels (r = 0.31 to 0.24, P < 0.02) as well as with hs-CRP and IL-6 (r = 0.52 and 0.29, P < 0.005) and negatively with HDL-cholesterol, adiponectin, and insulin sensitivity (r = −0.36 to −0.20, P < 0.02). With adjustments for percent body fat, correlations between E2 and metabolic risk variables were no longer significant. Similar results were observed for circulating estrone (E1) and estrone-sulfate (E1-S) levels. In conclusion, circulating estrogen concentrations are proportional to adipose mass in postmenopausal women, although they remain in the low range. Insulin resistance as well as altered blood lipids and cytokines are observed when circulating estrogen levels are high within that range, but these differences are explained by concomitant variation in total adiposity.
  • Publication
    Restreint
    Rapid restriction fragment analysis for screening four point mutations of the low-density lipoprotein receptor gene in French Canadians
    (Wiley-Liss, 1995-03-01) Simard, Jacques; Labrie, Fernand; Moorjani, Sital; Lupien, Paul-J.; Torres, Ana Lucia; Vohl, Marie-Claude; Gagné, Claude; Després, Jean-Pierre; Couture, Patrick
    Familial hypercholesterolemia (FH) has an estimated frequency of 1:154 among French Canadians in Northeastern Quebec, compared with 1:500 in most other populations. FH is caused by numerous mutations of the low-density lipoprotein (LDL) receptor gene, but only six well-characterized mutations are known to cause FH in French Canadians. High prevalence of the phenotype, along with a limited number of mutations in this population, provides a unique opportunity to study genotype-phenotype variation. Since the current methods for detection of point mutations in this population use complicated approaches, we report polymerase chain reaction (PCR)-based restriction fragment analysis to detect all four point mutations. This approach provides a rapid diagnosis and is suitable to screen large number of samples for studies in genetic epidemiology; it should be useful in identifying FH in other populations bearing the same mutations.
  • Publication
    Restreint
    Identification of three mutations in the low-density lipoprotein receptor gene causing familial hypercholesterolemia among French Canadians
    (Wiley-Liss, 2011-04-28) Simard, Jacques; Labrie, Fernand; Gagné, Claude; Lupien, Paul-J.; Gaudet, Daniel; Torres, Ana Lucia; Vohl, Marie-Claude; Després, Jean-Pierre; Couture, Patrick; Moorjani, Sital
  • Publication
    Accès libre
    Aspects of the mechanism of action of hypothalamic releasing hormones in the anterior pituitary gland
    (Urban & Schwarzenberg, 1975-01-01) Barden, Nicholas; Pelletier, Georges; Labrie, Fernand