Personne : Hoesli, Corinne A.
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Université Laval. Département de génie des mines, de la métallurgie et des matériaux
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- PublicationRestreintAtmospheric pressure plasma polymer of ethyl lactate: In vitro degradation and cell viability studies(Wiley, 2016-03-29) Laroche, Gaétan; Koehler, Julia; Hoesli, Corinne A.; Laurent, Morgane; Sabbatier, Gad; Ghérardi, NicolasEthyl lactate is injected into a dielectric barrier discharge (DBD) to build up a degradable plasma polymer (PP) to be used as a drug delivery system. Plasma power, deposition time, and type of carrier gas (Ar, N2) are correlated to the coating in vitro degradation rate. PPs are characterized by AFM, SEM, IR spectroscopy, XPS, and SEC, while surface profilometry is used to monitor the degradation kinetics. PPs deposited under N2 are mainly composed of hydrophilic functionalities, which explain their fast degradation upon exposure to an aqueous environment. In contrast, PPs synthesized under Ar lead to a slower degradation rate due to their hydrocarbon structure containing some hydrolyzable moieties. The potential of the PPs for vascular applications is verified
- PublicationAccès libreHuman saphenous vein endothelial cell adhesion and expansion on micropatterned polytetrafluoroethylene(Wiley, 2012-08-31) Boivin, Marie-Claude; Laroche, Gaétan; Hoesli, Corinne A.; Lagueux, Jean; Bareille, Reine; Rémy-Zolghadri, Murielle; Chevallier, Pascale; Bordenave, Laurence; Durrieu, Marie-ChristineIntimal hyperplasia and thrombosis are responsible for the poor patency rates of small-diameter vascular grafts. These complications could be avoided by a rapid and strong adhesion of endothelial cells to the prosthetic surfaces, which typically consist of expanded polytetrafluoroethylene (PTFE) for small-diameter vessels. We have previously described two peptide micropatterning strategies that increase the endothelialization rates of PTFE. The micropatterns were generated either by inkjet printing 300 μm squares or by spraying 10.1 ± 0.1 μm diameter droplets of the CGRGDS cell adhesion peptide, while the remaining surface was functionalized using the CWQPPRARI cell migration peptide. We now directly compare these two micropatterning strategies and examine the effect of hydrodynamic stress on human saphenous vein endothelial cells grown on the patterned surfaces. No significant differences in cell adhesion were observed between the two micropatterning methods. When compared to unpatterned surfaces treated with a uniform mixture of the two peptides, the cell expansion was significantly higher on sprayed or printed surfaces after 9 days of static cell culture. In addition, after 6 h of exposure to hydrodynamic stress, the cell retention and cell cytoskeleton reorganization on the patterned surfaces was improved when compared to untreated or random treated surfaces. These results indicate that micropatterned surfaces lead to improved rates of PTFE endothelialization with higher resistance to hydrodynamic stress.
- PublicationAccès libreDynamics of endothelial cell responses to laminar shear stress on surfaces functionalized with fibronectin-derived peptides(American Chemical Society, 2018-10-11) Duchesne, Carl; Ruel, Jean; Tremblay, Catherine; Juneau, Pierre-Marc; Beland, Ariane V.; Garnier, Alain; Ling, Si Da; Boulanger, Mariève D.; Laroche, Gaétan; Hoesli, Corinne A.; Gaillet, BrunoSurface endothelialization could improve the long-term performance of vascular grafts and stents. We previously demonstrated that aerosol-generated fibronectin-derived peptide micropatterns consisting of GRGDS spots over a WQPPRARI background increase endothelial cell yields in static cultures. We developed a novel fluorophore-tagged RGD peptide (RGD-TAMRA) to visualize cell–surface interactions in real-time. Here, we studied the dynamics of endothelial cell response to laminar flow on these peptide-functionalized surfaces. Endothelial cells were exposed to 22 dyn/cm² wall shear stress while acquiring time-lapse images. Cell surface coverage and cell alignment were quantified by undecimated wavelet transform multivariate image analysis. Similar to gelatin-coated surfaces, surfaces with uniform RGD-TAMRA distribution led to cell retention and rapid cell alignment (∼63% of the final cell alignment was reached within 1.5 h), contrary to the micropatterned surfaces. The RGD-TAMRA peptide is a promising candidate for endothelial cell retention under flow, and the spray-based micropatterned surfaces are more promising for static cultures.
- PublicationAccès libreSurface grafting of Fc-binding peptides as a simple platformto immobilize and identify antibodies that selectively capture circulating endothelial progenitor cells(2020-09-09) Bashth, Omar S.; Laroche, Gaétan; Elkhodirya, Mohamed A.; Hoesli, Corinne A.Antibody surface immobilization is a promising strategy to capture cells of interest from circulating fluids in vitro and in vivo. An application of particular interest in vascular interventions is to capture endothelial progenitor cells (EPCs) on the surface of stents to accelerate endothelialization. The clinical impact of EPC capture stents has been limited by the lack of efficient selective cell capture. Here, we describe a simple method to immobilize a variety of immunoglobulin G antibodies through their fragment crystallizable (Fc) regions via surface-conjugated RRGW peptides for cell capture applications. As an EPC capture model, peripheral blood endothelial colony-forming cells suspended in cell culture medium with up to 70% serum were captured by immobilized anti-CD144, anti-CD34 or anti-CD309 antibodies under laminar flow. The endothelial colony-forming cells were successfully enriched from a mixture with peripheral blood mononuclear cells using surfaces with anti-CD309 but not anti-CD45. This antibody immobilization approach holds great promise to engineer vascular biomaterials with improved EPC capture potential. The ease of immobilizing different antibodies using the same Fc-binding peptide surface grafting chemistry renders this platform suitable to screen antibodies that maximize cell capture efficiency and selectivity.