Personne :
Turgeon, Sylvie

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Turgeon
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Sylvie
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Université Laval. Département des sciences des aliments
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ncf10325454
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Voici les éléments 1 - 10 sur 17
  • Publication
    Accès libre
    Seaweeds : a traditional ingredients for new gastronomic sensation
    (Boca Raton : CRC Press, 2017-02-08) Rioux, Laurie-Eve; Beaulieu, Lucie; Turgeon, Sylvie
    Seaweeds have a long tradition in Asian cuisine. In Canada and US, seaweed consumption is mostly limited to sushi and other imported Asian dish. However, seaweeds are well recognized for their richness in several nutrients such as fiber, protein and minerals. But what is limiting seaweed and seaweed derived ingredients utilization in home cooking? Finding fresh seaweeds within inland cities is one limiting step but also the seaweed marketing need to propel the image that seaweed are not only nutritive but can bring flavor and texture in cuisine dish. With the rise of TV cooking shows, blogs and online recipes hosted by several renowned chefs, it is now time to bring seaweed in the spotlight. The aim of this review is to look at seaweeds to support a wider use in culinary applications for their nutritional contribution but also from a sensory perspective.
  • Publication
    Accès libre
    Effect of calcium on fatty acid bioaccessibility during in vitro digestion of Cheddar-type cheeses prepared with different milk fat fractions
    (Urbana, Ill. : American Dairy Science Association, 2017-03-18) Ayala-Bribiesca, Erik; Britten, Michel; Turgeon, Sylvie
    Calcium plays an important role in intestinal lipid digestion by increasing the lipolysis rate, but also limits fatty acid bioaccessibility by producing insoluble Ca soaps with long-chain fatty acids at intestinal pH conditions. The aim of this study was to better understand the effect of Ca on the bioaccessibility of milk fat from Cheddar-type cheeses. Three anhydrous milk fats (AMF) with different fatty acid profiles (olein, stearin, or control AMF) were used to prepare Cheddar-type cheeses, which were then enriched or not with Ca using CaCl2 during the salting step. The cheeses were digested in vitro, and their disintegration and lipolysis rates were monitored during the process. At the end of digestion, lipids were extracted under neutral and acidic pH conditions to compare free fatty acids under intestinal conditions in relation to total fatty acids released during the digestion process. The cheeses prepared with the stearin (the AMF with the highest ratio of long-chain fatty acids) were more resistant to disintegration than the other cheeses, owing to the high melting temperature of that AMF. The Ca-enriched cheeses had faster lipolysis rates than the regular Ca cheeses. Chromatographic analysis of the digestion products showed that Ca interacted with long-chain fatty acids, producing Ca soaps, whereas no interaction with shorter fatty acids was detected. Although higher Ca levels resulted in faster lipolysis rates, driven by the depletion of reaction products as Ca soaps, such insoluble compounds are expected to reduce the bioavailability of fatty acids by hindering their absorption. These effects on lipid digestion and absorption are of interest for the design of food matrices for the controlled release of fat-soluble nutrients or bioactive molecules.
  • Publication
    Accès libre
    Low-temperature blanching as a tool to modulate the structure of pectin in blueberry purees
    (Journal of food science, 2017-08-10) Chevalier, Laura; Rioux, Laurie-Eve; Angers, Paul.; Turgeon, Sylvie
    Blueberry composition was characterized for 6 cultivars. It contains a good amount of dietary fiber (10% to 20%) and pectin (4% to 7%) whose degree of methylation (DM) is sensitive to food processing. A low temperature blanching (LTB: 60 °C/1 h) was applied on blueberry purees to decrease pectin DM, in order to modulate puree properties and functionalities (that is, viscosity and stability), and to enhance pectin affinity toward other components within food matrices. Fiber content, viscosity, pectin solubility, DM, and monosaccharide composition were determined for both pasteurized, and LTB+pasteurized blueberry purees. The results showed that neither the amount of fiber, nor the viscosity were affected by LTB, indicating that this treatment did not result in any significant pectin depolymerization and degradation. LTB caused a decrease both in pectin DM from 58–67% to 45–47% and in the amount of water-soluble pectin fraction, the latter remaining the major fraction of total pectin at 52% to 57%. A LTB is a simple and mild process to produce blueberry purees with mostly soluble and low-methylated pectin in order to extend functionality and opportunities for interactions with other food ingredients.
  • Publication
    Accès libre
    Effect of calcium enrichment of Cheddar cheese on its structure, in vitro digestion and lipid bioaccessibility
    (Oxford : Elsevier Ltd., 2015-09-28) Ayala-Bribiesca, Erik; Lussier, Martine; Chabot, Denise; Turgeon, Sylvie; Britten, Michel
    The nutritional role of cheese is usually reduced to its composition, often neglecting the effect that the matrix can have on digestion. The purpose of this study was to establish a link between the characteristics of Cheddar cheeses with different calcium levels and the impact on cheese in vitro digestion. Curds were enriched with CaCl2 during the salting step to produce control, high-calcium, and very high-calcium cheeses. Cheese composition, texture and structure were characterized, and physical disintegration and lipolysis were monitored during in vitro digestion. Cheese hardness increased with higher calcium content. This resulted in a slower disintegration during in vitro digestion. Despite showing faster disintegration, the control cheese had the slowest lipolysis progression. The results suggest that lipolysis depends on calcium content and the matrix modulating the access of enzymes to their substrates. Further studies should provide a better understanding of the calcium–matrix interaction affecting lipid bioaccessibility.
  • Publication
    Accès libre
    Quantitative PCR reveals the frequency and distribution of 3 indigenous yeast species across a range of specialty cheeses
    (American Dairy Science Association, 2022-09-14) Lamarche, Andréanne; Lessard, Marie-Hélène; Viel, Catherine; Turgeon, Sylvie; St-Gelais, Daniel; Labrie, Steve
    Indigenous microorganisms are important components of the complex ecosystem of many dairy foods including cheeses, and they are potential contributors to the development of a specific cheese's sensory properties. Among these indigenous microorganisms are the yeasts Cyberlindnera jadinii, Pichia kudriavzevii, and Kazachstania servazzii, which were previously detected using traditional microbiological methods in both raw milk and some artisanal specialty cheeses produced in the province of Québec, Canada. However, their levels across different cheese varieties are unknown. A highly specific and sensitive real-time quantitative PCR assay was developed to quantitate these yeast species in a variety of specialty cheeses (bloomy-rind, washed-rind, and natural-rind cheeses from raw, thermized, and pasteurized milks). The specificity of the quantitative PCR assay was validated, and it showed no cross-amplification with 11 other fungal microorganisms usually found in bloomy-rind and washed-rind cheeses. Cyberlindnera jadinii and P. kudriavzevii were found in the majority of the cheeses analyzed (25 of 29 and 24 of 29 cheeses, respectively) in concentrations up to 104 to 108 gene copies/g in the cheese cores, which are considered oxygen-poor environments, and 101 to 104 gene copies/cm2 in the rind. However, their high abundance was not observed in the same samples. Whereas C. jadinii was present and dominant in all core and rind samples, P. kudriavzevii was mostly present in cheese cores. In contrast, K. servazzii was present in the rinds of only 2 cheeses, in concentrations ranging from 101 to 103 gene copies/cm2, and in 1 cheese core at 105 gene copies/g. Thus, in the ecosystems of specialty cheeses, indigenous yeasts are highly frequent but variable, with certain species selectively present in specific varieties. These results shed light on some indigenous yeasts that establish during the ripening of specialty cheeses.
  • Publication
    Restreint
    Peptides from milk protein hydrolysates to improve the growth of human keratinocytes in culture
    (Elsevier Applied Science, 2004-07-01) Amiot, Jean; Germain, Lucie; Auger, François A.; Ory-Salam, Christine; Lemay, Martine; Turgeon, Sylvie
    Milk and colostrum are known to contain constituents having growth promoting activities on various human cell lines. Peptides from milk protein hydrolysates have also been shown to have various nutraceutical and biological properties. The aim of this research was to establish conditions for the in vitro hydrolysis of milk proteins and for the separation and identification of peptides that could promote growth of human skin cells in culture. Milk protein hydrolysates were obtained by using trypsin and chymotrypsin with various enzyme to substrate ratios (E/S) and degrees of hydrolysis (DH). Peptides contained in a 6% DH hydrolysate obtained with chymotrypsin were separated by size exclusion chromatography and supplemented in in vitro culture media to measure their efficacy to promote growth of keratinocytes isolated from human skin. The results indicated that growth promoting activity was increased up to 108% for keratinocytes cultured in medium supplemented with 300 μg mL−1 of one peptide fraction. Fifteen peptides isolated from this fraction by RP-HPLC and characterized by Pico-Tag amino acids analysis were shown to have an average molecular weight of 800 Da and to contain high concentrations of hydrophobic and aromatic amino acids.
  • Publication
    Accès libre
    Disintegration and nutrients release from cheese with different textural properties during in vitro digestion
    (New York : Elsevier, 2016-09-12) Fang, Xixi; Rioux, Laurie-Eve; Labrie, Steve; Turgeon, Sylvie
    Recent results showed that solid food disintegration in the stomach may be affected by food texture which was demonstrated to change during digestion. Cheese is complex as, depending on the variety, its composition and texture can be modulated. Cheddar, light Cheddar, Mozzarella and light Mozzarella cheese particles were digested in vitro. Cheese disintegration and nutrients release were studied throughout the oral, gastric and duodenal digestion steps in presence or absence of enzymes. Cheese disintegration was significantly affected by the enzymatic treatment (with or without enzymes). The addition of enzymes allowed to reach 72% of cheese disintegration at the end of the duodenal digestion while it has attained 30% when no enzymes were added. Cheddar cheese disintegration was the highest among cheeses. This phenomenon was related to its initial higher fat content which resulted in a higher fat release during digestion. The disintegration at the end of each digestion step was also correlated to cheese composition (proteolysis and fat) and to textural parameters (hardness, resilience, adhesiveness and chewiness). Light Cheddar and Mozzarella exhibited similar disintegration and nutrients release at the end of the digestion due to a relatively small fat reduction (6%) which had limited effect on cheese texture. This study provides quantitative evidence regarding the impact of cheese textural changes during digestion on cheese disintegration and macronutrients release which may further affect nutrients anabolic response and some physiological functions.
  • Publication
    Accès libre
    Impact of starch and exopolysaccharide-producing lactic acid bacteria on the properties of set and stirred yoghurts
    (New York (N.Y.) : Elsevier, 2016-01-21) Gentès, Marie-Claude; Turgeon, Sylvie; St-Gelais, Daniel
    The impact of exopolysaccharide (EPS)-producing lactic acid bacteria with well-known structures and starch (0.75%) on the rheological properties (apparent viscosity and elastic modulus) and physical properties (syneresis) of set and stirred yoghurts was studied. Three EPS-producing strains with different structural characteristics were studied: Streptococcus thermophilus ST1 (anionic, stiff and linear EPS), Lactobacillus delbrueckii subsp. bulgaricus LB1 (neutral, stiff and ramified EPS) and Lb. delbrueckii subsp. bulgaricus LB2 (neutral, flexible and highly ramified EPS). The presence of linear, stiff, and anionic EPS from ST1 increased the elastic modulus in all yoghurt conditions, possibly owing to electrostatic interactions with caseins. Higher viscosity values were obtained with stiff and linear or slightly branched EPS from the ST1 and LB1 for all yoghurt conditions. Starch addition increased the values of the rheological and physical properties of all stirred yoghurts, probably due to the repulsion between proteins and polysaccharides favouring thermodynamic incompatibility.
  • Publication
    Accès libre
    Postprandial lipemia and fecal fat excretion in rats is affected by the calcium content and type of milk fat present in Cheddar-type cheeses
    (New York, NY : Elsevier Science, 2018-03-21) Ayala-Bribiesca, Erik; Turgeon, Sylvie; Pilon, Geneviève; Britten, Michel; Marette, André.
    The aim of this study was to better understand the effect of calcium on the bioavailability of milk lipids from a cheese matrix using a rat model. Cheddar-type cheeses were manufactured with one of three types of anhydrous milk fat, control, olein or stearin, and salted with or without CaCl₂. The cheeses were fed to rats and postprandial lipemia was monitored. Feces were analyzed to quantify fatty acids excreted as calcium soaps. Higher calcium concentration in cheese caused a higher and faster triacylglycerol peak in blood, except for cheeses containing stearin. Furthermore, calcium soaps were more abundant in feces when the ingested cheese had been enriched with calcium and when the cheese was prepared with stearin. Increased lipid excretion was attributable to the affinity of saturated long-chain fatty acids for calcium. Results showed that lipid bioaccessibility can be regulated by calcium present in Cheddar cheese. This study highlights the nutritional interaction between calcium and lipids present in the dairy matrix and confirms its physiological repercussions on fatty acid bioavailability.
  • Publication
    Accès libre
    Formation and functional properties of protein–polysaccharide electrostatic hydrogels in comparison to protein or polysaccharide hydrogels
    (New York, NY : Elsevier Science Pub. Co., 2017-02-16) Le, Xuan Thang; Rioux, Laurie-Eve; Turgeon, Sylvie
    Protein and polysaccharide mixed systems have been actively studied for at least 50 years as they can be assembled into functional particles or gels. This article reviews the properties of electrostatic gels, a recently discovered particular case of associative protein–polysaccharide mixtures formed through associative electrostatic interaction under appropriate solution conditions (coupled gel). This review highlights the factors influencing gel formation such as protein–polysaccharide ratio, biopolymer structural characteristics, final pH, ionic strength and total solid concentration. For the first time, the functional properties of protein–polysaccharide coupled gels are presented and discussed in relationship to individual protein and polysaccharide hydrogels. One of their outstanding characteristics is their gel water retention. Up to 600 g of water per g of biopolymer may be retained in the electrostatic gel network compared to a protein gel (3–9 g of water per g of protein). Potential applications of the gels are proposed to enable the food and non-food industries to develop new functional products with desirable attributes or new interesting materials to incorporate bioactive molecules.