Personne : Laroche, Gaétan
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Université Laval. Département de génie des mines, de la métallurgie et des matériaux
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- PublicationAccès libreAtmospheric pressure cold plasma versus wet-chemical surface treatments for carboxyl functionalization of polylactic acid : a first step toward the immobilization of bioactive molecules(Elsevier, 2020-02-08) Laroche, Gaétan; Rodríguez Durán, Iván; Vanslambrouck, Stéphanie; Chevallier, PascaleThe use of polylactic acid (PLA) has attracted growing interest, particularly in recent years, for biomedical applications because of its mechanical properties, biocompatibility, and biodegradability. Despite this, features such as surface hydrophobicity and the absence of suitable functional groups for covalent immobilization of bioactive molecules, make it challenging to endow PLA-based medical devices with additional features and thus broaden their range of applicability. In the present study, we demonstrate the suitability of atmospheric pressure dielectric barrier discharges operating in the Townsend regime as a promising alternative to other surface treatments, such as diazonium and alkali hydrolytic treatments, for carboxyl functionalization of PLA. Chemical changes in PLA surfaces are evaluated by contact angle measurements and by X-ray photoelectron spectroscopy while physical changes are investigated by scanning electron microscopy and atomic force microscopy. The amount of carboxyl groups generated on PLA surfaces is assessed by toluidine blue O assay and substantiated by grafting, through carboxyl groups, a fluorescent probe containing amino functionalities. All of the surface treatments have proven to be very effective in generating carboxylic groups on the PLA surface. Nevertheless, plasma treatment is shown to not degrade the PLA surface, in sharp contrast with diazonium and alkali hydrolytic treatments.
- PublicationAccès libreA new approach for synthesizing plasmonic polymer nanocomposite thin films by combining a gold salt aerosol and an atmospheric pressure low-temperature plasma(2021-02-05) Nadal, Elie; Milaniak, Natalia; Laroche, Gaétan; Glenat, Hervé; Massines, FrançoiseThe proof of the concept of a new, onestep and safe by design process to synthesize metal-polymer nanocomposites thin films on a large surface is presented. It is based on the injection of an aerosol of a solution of metal (gold) salts dissolved in a polymerizable solvent (isopropanol) into an argon atmospheric pressure dielectric barrier discharge. The main novelty of this method resides in the fact that the nanoparticles are formed in situ, inside the plasma reactor, in the gas phase. Consequently, the nanoparticle synthesis and deposition are concomitant with the solvent polymerization used to produce the matrix, which makes it possible to obtain homogeneous layers of non-agglomerated nanoparticles (NPs) with high NPs density. By toggling between low and high-frequency discharges, gold/polymer nanocomposites with different morphologies and optical properties are synthesized. The effect of the concentration of gold in the aerosol and the gas residence time in the plasma as well as the ratio of high and low-frequency discharge and their repetition rate are presented. The thin films are systematically characterized by AFM and UV–visible spectroscopy to analyze their morphologies along with their plasmonic resonances.
- PublicationAccès libreElectrode cleanliness impact on the surface treatment of fluoropolymer films for a long-lasting plasma process(Elsevier, 2020-09-16) Gélinas, Alex; Laroche, Gaétan; Laurent, MorganeA dielectric barrier discharge in a continuous process configuration is used to coat the surface of polymer films. The effect of the growth of a coating layer on top of the uncovered electrode with regards to the physicochemical properties of the film treatment is studied. Plasma electrical parameters such as power density (W.cm−2) and voltage (kV) are monitored during a typical cycle, which is comprised between two electrode cleanings. In addition, the surface energy and chemistry are determined at chosen time-points of the process by contact angle measurements with two liquids and X-ray photoelectron spectroscopy (XPS). Based on these analyses, no major modification of the coated polymer physicochemical properties was attributed to the deposition of an organic layer on top of the bare electrodes after the equivalent of 1 h of continuous treatment.
- PublicationAccès libreUnveiling the origin of the anti fogging of plasma-coated glass : role of the structure and chemistry of siloxane precursors(Elsevier Science, 2020-01-25) Laroche, Gaétan; Rodríguez Durán, IvánThe application of (super)hydrophilic coatings in sectors of activity concerned by fogging, such as the food industry, the architectural sector, and medicine has attracted enormous attention over the past few years. However, despite this interest, most of the coating deposition techniques used thus far are not suitable for large-scale production because of their multistep nature. In this regard, the use of atmospheric pressure dielectric barrier discharges (AP-DBD) operated under a controlled N2/N2O atmosphere offers a promising alternative to conventional deposition techniques for the fabrication of anti-fogging coatings. Using this one-step coating approach, four siloxane precursors with different structures and different number of Si―H and Si−CH3 groups; namely, 1,3,5,7-tetramethylcyclotetrasiloxane (TMCTS), octamethylcyclotetrasiloxane (OMCTS), 1,1,3,3-tetramethyldisiloxane (TMDSO), and hexamethyldisiloxane (HMDSO) were deposited on glass samples. Because of their extreme wetting behavior (WCA < 5°), TMCTS-coated glasses featured an excellent anti-fogging performance, in contrast to OMCTS-, TMDSO-, and HMDSO-coated glasses which were not fogging-resistant (WCA ≈ 80°)). Coupled with hydrophilic functionalities, such as Csingle bondO, Odouble bond Csingle bondO, and Sisingle bondOH groups, the relatively high surface roughness of TMCTS-coated glass, compared with that of OMCTS-, TMDSO-, or HMDSO-coated glass, accounted for its superior visual characteristics when exposed to water vapor at 80 °C. These results allow us to confidently conclude that the cyclic structure of TMCTS in conjunction with the high reactivity of the Si-H bonds is responsible for the observed anti-fogging effect.
- PublicationAccès libreSurface grafting of Fc-binding peptides as a simple platformto immobilize and identify antibodies that selectively capture circulating endothelial progenitor cells(2020-09-09) Bashth, Omar S.; Laroche, Gaétan; Elkhodirya, Mohamed A.; Hoesli, Corinne A.Antibody surface immobilization is a promising strategy to capture cells of interest from circulating fluids in vitro and in vivo. An application of particular interest in vascular interventions is to capture endothelial progenitor cells (EPCs) on the surface of stents to accelerate endothelialization. The clinical impact of EPC capture stents has been limited by the lack of efficient selective cell capture. Here, we describe a simple method to immobilize a variety of immunoglobulin G antibodies through their fragment crystallizable (Fc) regions via surface-conjugated RRGW peptides for cell capture applications. As an EPC capture model, peripheral blood endothelial colony-forming cells suspended in cell culture medium with up to 70% serum were captured by immobilized anti-CD144, anti-CD34 or anti-CD309 antibodies under laminar flow. The endothelial colony-forming cells were successfully enriched from a mixture with peripheral blood mononuclear cells using surfaces with anti-CD309 but not anti-CD45. This antibody immobilization approach holds great promise to engineer vascular biomaterials with improved EPC capture potential. The ease of immobilizing different antibodies using the same Fc-binding peptide surface grafting chemistry renders this platform suitable to screen antibodies that maximize cell capture efficiency and selectivity.
- PublicationAccès libreFourier-Transform infrared spectroscopy of ethyl lactate decomposition and thin-film coating in a filamentary and a glow dielectric barrier discharge(Wiley-VCH-Verl., 2021-07-05) Milaniak, Natalia; Laroche, Gaétan; Massines, FrançoiseGlow and filamentary regimes of atmospheric pressure plasma-enhanced chemical vapor deposition in a planar dielectric barrier discharge configuration were compared for thin-film deposition from ethyl lactate (EL). EL decomposition in the plasma phase and thin-film composition were both characterized by Fourier- transform infrared spectroscopy. EL chemical bonds' concentration along the gas flow decreases progressively in the glow dielectric barrier discharge (GDBD), whereas it drastically oscillates in the filamentary dielectric barrier discharge (FDBD), with values higher than that of the initial mixture. EL decomposition route depends on the discharge regime, as the decrease of the concentration of the different investigated bonds is different for an identical amount of energy provided to EL molecules. CO2 is systematically formed reaching concentrations of 25 and 40 ppm, respectively, in FDBD and GDBD.
- PublicationAccès librePolycaprolactone (PCL) chains grafting on the surface of cellulose nanocrystals (CNCs) during in situ polymerization of ε-caprolactone at room temperature(Materials Sciences and Applications, Vol. 11 (11), 2020, 2020-11-13) Astruc, jérémy; Cousin, Patrice; Laroche, Gaétan; Robert, Mathieu; Elkoun, S. (Saïd)This work aimed at investigating the feasibility of surface modification of cellulose nanocrystals (CNCs) using in situ ring opening polymerization of ε-caprolactone (ε-CL) at room temperature. Residues of flax and milkweed (Asclepias syriaca) stem fibers were used as a source of cellulose to obtain and isolate CNCs. The cationic ring opening polymerization (CROP) of the monomer ε-CL was used to covalently graft polycaprolactone (PCL) chains at the CNCs surface. Silver hexafluoroantimonate (AgSbF6) was used in combination with the extracted CNCs to initiate, at room temperature, the polymerization and the grafting reactions with no other stimulus. Fourier-Transform InfraRed (FTIR), X-ray Photoelectron Spectrometry (XPS), UV/visible absorption and Gel Permeation Chromatography (GPC) analyses evidenced the presence of PCL chains covalently grafted at CNCs surface, the formation of Ag(0) particles as well as low or moderate molecular weight free PCL chains.
- PublicationAccès libreFibronectin grafting to enhance skin sealing around transcutaneous titanium implant(John Wiley & Sons, 2021-04-30) Bilem, Ibrahim; Ghadhab, Souhaila; Ruel, Jean; Laroche, Gaétan; Auger, François A.; Guay-Bégin, Andrée-Anne; Pauthe, Emmanuel; Chevallier, PascaleIntraosseous transcutaneous amputation prosthesis is a new approach in orthopedic implants that overcomes socket prosthesis problems. Its long-term performance requires a tight skin-implant seal to prevent infections. In this study, fibronectin (Fn), a widely used adhesion protein, was adsorbed or grafted onto titanium alloy. Fn grafting was performed using two different linking arms, dopamine/glutaric anhydride or phosphonate. The characterization of Fn-modified surfaces showed that Fn grating via phosphonate has led to the highest amount of Fn cell-binding site (RGD, arginine, glycine, and aspartate) available on the surface. Interestingly, cell culture studies revealed a strong correlation between the amount of available RGD ligands and cellular behavior, since enhanced proliferation and spreading of fibroblasts were noticed on Fn-grafted surfaces via phosphonate. In addition, an original in vitro mechanical test, inspired from the real situation, to better predict clinical outcomes after implant insertion, has been developed. Tensile test data showed that the adhesion strength of a bio-engineered dermal tissue was significantly higher around Fn-grafted surfaces via phosphonate, as compared to untreated surfaces. This study sheds light on the importance of an appropriate selection of the linking arm to tightly control the spatial conformation of biomolecules on the material surface, and consequently cell interactions at the interface tissue/implant.
- PublicationRestreintAtmospheric-pressure plasma-enhanced chemical vapor deposition of nanocomposite thin films from ethyl lactate and silica nanoparticles(Weinheim Wiley-VCH-Verl., 2020-10-09) Milaniak, Natalia; Laroche, Gaétan; Massines, FrançoiseNanocomposite coatings are made by atmospheric-pressure plasma-enhanced chemical vapor deposition from ethyl lactate (EL) and silica nanoparticles (NPs) in a dielectric barrier discharge (DBD) using frequency-shift keying (FSK) to alternate between 1- and 15-kHz voltages. In situ plasma Fourier-transform infrared spectroscopy (FTIR) and thin film FTIR, scanning electron microscopy, atomic force microscopy, and profilometry show that (i) 1 kHz DBD mainly deposits NPs, 15 kHz only polymerizes EL; (ii) the EL polymerization rate is the same in FSK and continuous modes; (iii) despite the 50/50 contribution of both frequencies, the NP deposit is three times faster in FSK mode than in 1 kHz DBD and compared with 1 and 15 kHz coatings, in the nanocomposite, NP Si–O–Si and EL C═O bonds per unit length are equal to 68% and 34%, respectively. In situ FTIR detects SiO2 NPs, their functionalization, and the formation of CO2.
- PublicationAccès libreResponse surface methodology as a predictive tool for the fabrication of coatings with optimal anti-fogging performance(Lausanne Elsevier Sequoia, 2020-12-15) Laroche, Gaétan; Profili, Jacopo; Rodríguez Durán, Iván; Stafford, LucIn this study, response surface methodology was applied to optimize the anti-fogging performance of coatings deposited on commercial glass samples from 1,3,5,7-tetramethylcyclotetrasiloxane (TMCTS)/N2O mixtures by atmospheric pressure plasma enhanced chemical vapor deposition. The effect of the dissipated power (DP), [N2O]/[TMCTS] ratio, and sample scroll speed on the anti-fogging performance was investigated by means of a Box-Behnken experimental design. The regression model relating transmittance of the coated glasses to these deposition parameters revealed that the anti-fogging performance strongly depends on the second-order interaction of the dissipated power and [N2O]/[TMCTS] ratio (i.e., DP × [N2O]/[TMCTS]). Contour plots showed that the dissipated power required to prepare optimal anti-fogging coatings should be of at least 0,7, 0,5 or 0,4 W cm−2, if the [N2O]/[TMCTS] ratio in the plasma is 20, 30, or 40, respectively. When placed over water at 50°C, the coated glass samples allowed 80% (or more) of 590-nm light to pass through, thus meeting the minimal anti-fogging requirement for alpine skier goggles and faceshields. Despite not having a significant impact on the anti-fogging performance, the sample scroll speed is key to fabricating coatings with the desired thickness during in-line manufacturing.