Personne :
Gagné, Dominic

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Université Laval. Département de biologie
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Voici les éléments 1 - 3 sur 3
  • PublicationAccès libre
    Positive modulation of meat microbial ecology by feeding strategies
    (2016-08-14) Kone, Amenan Prisca Nadege; Gagné, Dominic; Cinq-Mars, Dany; Saucier, Linda; Guay, Frédéric
    The objective of this work was to determine if it is possible to modulate carcass/meat contamination by feeding farm animals with a diet supplemented with a protective culture, here Carnobacterium maltaromaticum CB1/UAL307 producing three bacteriocins, including carnocyclin A. A total of 144 weaned Grimaud female rabbits (a commercial meat breed) were divided into two feeding groups: 1) a control commercial diet and 2) the same diet supplemented with Micocin®, a commercial preparation of C. maltaromaticum CB1, at a final concentration of 8-log CFU (Colony Forming Unit) per kg of feed. Rabbits were fed their respective diet until they reached a commercial slaughter weight of 2.2 kg. Presence of C. maltaromaticum producing carnocyclin A was confirmed by PCR amplification for three specific genes. Its prevalence was greater in faeces, on thighs and in ground meat from rabbits fed the ration supplemented with Micocin®. These results demonstrate that the microflora of the feed can influence the organisms contaminating the end products and emphasized the importance of providing farm animals with high microbial quality feed and hygienic conditions to grow.
  • PublicationAccès libre
    Application of Carnobacterium maltaromaticum as a feed additive for weaned rabbits to improve meat microbial quality and safety
    (Applied Science Publishers., 2017-10-02) Kone, Amenan Prisca Nadege; Zea, Juliana Maria Velez; Gagné, Dominic; Cinq-Mars, Dany; Saucier, Linda; Guay, Frédéric
    This study addresses the improvement of meat microbial quality by enriching the diet of farm animals with a protective culture. Weaned Grimaud rabbits were divided into two experimental groups: a control and a diet supplemented with Micocin® (Carnobacterium maltaromaticum CB1; 8 Log10 CFU/kg of feed). Overall, meat quality was not affected substantially by the treatment. Total Aerobic Mesophilic (TAM), Escherichia coli and other coliforms, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas spp., Listeria spp. and presumptive lactic acid bacteria counts were evaluated on whole thighs stored under aerobic (0, 3, 6, 8 days) and anaerobic (0, 5, 10, 15, 20 days) conditions at 4 °C. The results demonstrated that the microflora on refrigerated thighs was modulated by the addition of Micocin® (P < 0.05) and that the most effective reduction of Listeria monocytogenes growth was observed with ground meat stored under anaerobic conditions at 4 °C with a 2 Log difference at the end of a 15-day storage (P = 0.025).
  • PublicationRestreint
    Cumulus cell transcripts transit to the bovine oocyte in preparation for maturation
    (Society for the Study of Reproduction., 2015-11-13) Gilbert, Isabelle; Ettaoumi, Sara; Bastien, Alexandre; Gagné, Dominic; Ashkar, Fazl; Khandjian, Edward William; Macaulay, Angus; Shojaei Saadi, Habib Allah; Richard, François J.; Robert, Claude; Fournier, Éric; Sirard, Marc-André; Hyttel, P.
    So far, the characteristics of a good quality egg have been elusive, similar to the nature of the physiological, cellular, and molecular cues leading to its production both in vivo and in vitro. Current understanding highlights a strong and complex interdependence between the follicular cells and the gamete. Secreted factors induce cellular responses in the follicular cells, and direct exchange of small molecules from the cumulus cells to the oocyte through gap junctions controls meiotic arrest. Studying the interconnection between the cumulus cells and the oocyte, we previously demonstrated that the somatic cells also contribute transcripts to the gamete. Here, we show that these transcripts can be visualized moving down the transzonal projections (TZPs) to the oocyte, and that a time course analysis revealed progressive RNA accumulation in the TZPs, indicating that RNA transfer occurs before the initiation of meiosis resumption under a timetable fitting with the acquisition of developmental competence. A comparison of the identity of the nascent transcripts trafficking in the TZPs, with those in the oocyte increasing in abundance during maturation, and that are present on the oocyte's polyribosomes, revealed transcripts common to all three fractions, suggesting the use of transferred transcripts for translation. Furthermore, the removal of potential RNA trafficking by stripping the cumulus cells caused a significant reduction in maturation rates, indicating the need for the cumulus cell RNA transfer to the oocyte. These results offer a new perspective to the determinants of oocyte quality and female fertility, as well as provide insight that may eventually be used to improve in vitro maturation conditions.