Personne :
Bazinet, Laurent

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Bazinet
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Laurent
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Université Laval. Département des sciences des aliments
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ncf10380374
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2012 - 20173
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  • Publication
    Accès libre
    Inhibitory effects of commercial and enriched green tea extracts on the growth of Brochothrix thermosphacta, Pseudomonas putida and Escherichia coli
    (Canadian Center of Science and Education., 2012-11-21) Rozoy, Elodie; Guernec, Anthony; Bazinet, Laurent; Araya-Farias, Mónica.; Saucier, Linda
    The major catechin found in green tea, called epigallocatechin gallate (EGCG), have been reported to have antimicrobial properties. In this study, we examined in vitro the antimicrobial effects of a commercial green tea extract sold in a capsule form, and two prepared green tea extracts enriched in catechins against Brochothrix thermosphacta, Pseudomonsas putida and Escherichia coli which have been associated with meat spoilage. The antimicrobial activity of the different tea extracts was evaluated by Spot-On-Lawn and Well Diffusion assays and the Minimum Inhibitory Concentration (MIC) was also determined in Brain Heart Infusion broth. The three methods used showed an inhibition of Brochothrix thermosphacta, whereas the inhibition of Pseudomonas putida and Escherichia coli was only detected with the MIC assay. The determination of the MIC in broth culture appeared to be the most reliable method to determine the inhibitory activity of catechin compounds.
  • Publication
    Accès libre
    Fouling prevention of peptides from a tryptic whey hydrolysate during electromembrane processes by use of monovalent ion permselective membranes
    (Elsevier, 2017-12-13) Persico, Mathieu; Bazinet, Laurent
    Peptide adsorption occurring on conventional anion- and cation-exchange membranes is one of the main technological locks in electrodialysis (ED) for hydrolysate demineralization. Hence, the peptide fouling of monovalent anion (MAP) and monovalent cation (MCP) permselective membranes was studied and compared to conventional membranes (AMX-SB and CMX-SB). It appeared that the main peptide sequences responsible for fouling were TPEVDDEALEKFDK, VAGTWY and VLVLDTDYK for both anionic membranes; and ALPMHIR and TKIPAVFK for both cationic membranes. However based on the MS-MS results, the fouling was about 97–100% lower for MAP than AMX-SB and 95–100% lower for MCP than CMX-SB. This was explained by the differences in charge sign distribution at the membrane surface. Consequently, monovalent membranes can represent a very interesting opportunity for treatment of hydrolysate solution in electrodialytic processes by practically eliminating peptide fouling. At our knowledge, it was the first time that such a demonstration was done.
  • Publication
    Accès libre
    Milk protein production by a more environmentally sustainable process : bipolar membrane electrodialysis coupled with ultrafiltration
    (Royal Society of Chemistry, 2017-11-28) Bazinet, Laurent; Patouillard, Laure; Mikhaylin, Sergey; Margni, Manuele
    The increased demand for food production to nourish the rapidly growing human population raises serious sustainability issues for the food sector. Indeed, conventional food production lines involve processes having a significant environmental burden. Hence, the present study aims to demonstrate an environmentally sustainable process of food production. The milk protein was chosen as a model food ingredient due to its exceptional role in the human diet. The proposed innovative process of milk protein production includes bipolar membrane electrodialysis coupled with ultrafiltration (EDBM-UF). The crucial problem during the EDBM-UF of milk, such as different types of membrane fouling, was successfully solved. Moreover, the life cycle assessment of the novel EDBM-UF protein production process was carried out and compared to a conventional acid/base process. Additionally, a sensitivity test of electricity supply at different geographical locations of the world was performed since electricity is the main energy source for the EDBM-UF process and it could be derived from different sources (renewable and non-renewable). The assessment results demonstrate that the proposed electromembrane process has significant environmental benefits compared to the conventional process using chemicals independently from the electricity supply mix from all considered geographical locations. Thus, EDBM-UF could become a prospective industrial technology taking into account environmental concerns and promoting the development of healthy human society.