Personne :
Lefèvre, Thierry

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Adresse électronique
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Structures organisationnelles
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Université Laval. Département de chimie
Identifiant Canadiana

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  • PublicationRestreint
    A surface spectroscopy study of a Pseudomonas fluorescens biofilm in the presence of an immobilized air bubble
    (Elsevier, 2019-05-28) Lefèvre, Thierry; Greener, Jesse; Abbaszadeh Amirdehi, Mehran; Pousti, Mohammad
    A linear spectral mapping technique was applied to monitor the growth of biomolecular absorption bands at the bio-interface of a nascent Pseudomonas fluorescens biofilm during and after interaction with a surface-adhered air bubble. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectra were obtained in different locations in a microchannel with adequate spatial and temporal resolution to study the effect of a static bubble on the evolution of protein and lipid signals at the ATR crystal surface. The results reveal that the presence of a bubble during the lag phase modified levels of extracellular lipids and affected a surface restructuring process, many hours after the bubble's disappearance.