Personne :
Guillemette, Maxime.

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Structures organisationnelles
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Département de physique, de génie physique et d'optique, Faculté des sciences et de génie, Université Laval
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Voici les éléments 1 - 5 sur 5
  • Publication
    Tissue-Engineered Vascular Adventitia with Vasa Vasorum Improves Graft Integration and Vascularization Through Inosculation
    (2010-05-05) Guillemette, Maxime.; Perron, Cindy; Germain, Lucie; Labbé, Raymond; Auger, François A.; Gauvin, Robert
    Tissue-engineered blood vessel is one of the most promising living substitutes for coronary and peripheral artery bypass graft surgery. However, one of the main limitations in tissue engineering is vascularization of the construct before implantation. Such a vascularization could play an important role in graft perfusion and host integration of tissue-engineered vascular adventitia. Using our self-assembly approach, we developed a method to vascularize tissue-engineered blood vessel constructs by coculturing endothelial cells in a fibroblast-laden tissue sheet. After subcutaneous implantation, enhancement of graft integration within the surrounding environment was noted after 48 h and an important improvement in blood circulation of the grafted tissue at 1 week postimplantation. The distinctive branching structure of end arteries characterizing the in vivo adventitial vasa vasorum has also been observed in long-term postimplantation follow-up. After a 90-day implantation period, hybrid vessels containing human and mouse endothelial cells were still perfused. Characterization of the mechanical properties of both control and vascularized adventitia demonstrated that the ultimate tensile strength, modulus, and failure strain were in the same order of magnitude of a pig coronary artery. The addition of a vasa vasorum to the tissue-engineered adventitia did not influence the burst pressure of these constructs. Hence, the present results indicate a promising answer to the many challenges associated with the in vitro vascularization and in vivo integration of many different tissue-engineered substitutes.
  • Publication
    Accès libre
    Recent advances in the development of tissue-engineered vascular media made by self-assembly
    (Elsevier, 2013-06-05) Guillemette, Maxime.; Laterreur, Véronique; Germain, Lucie; Ruel, Jean; Miville Godin, Caroline; Bourget, Jean-Michel; Mounier, Maxence; Veres, Teodor; Auger, François A.; Gauvin, Robert
    There is a lack of an optimal transplant material for small calibre blood vessels. This could be overcome by tissue engineering. The optimal construct is to be derived from autologous cells and present mechanical resistance comparable to the gold standard, autologous vessels such as the internal mammary artery or the saphenous vein. Our laboratory has developed the self-assembly approach to produce tissue sheets that can be rolled into such vessel substitutes. Over the years, many improvements have been made to the technique to facilitate smooth muscle cell culture and to produce vascular media substitutes with higher circumferential mechanical resistance.
  • Publication
    Surface topography induces 3D self-orientation of cells and extracellular matrix resulting in improved tissue function
    (RSC Pub., 2009-01-15) Guillemette, Maxime.; Cui, Bo; Germain, Lucie; Roy, Emmanuel; Veres, Teodor; Auger, François A.; Giasson, Claude J.; Gauvin, Robert; Esch, Mandy B.; Carrier, Patrick; Deschambeault, Alexandre; Dumoulin, Michel; Toner, Mehmet
    The organization of cells and extracellular matrix (ECM) in native tissues plays a crucial role in their functionality. However, in tissue engineering, cells and ECM are randomly distributed within a scaffold. Thus, the production of engineered-tissue with complex 3D organization remains a challenge. In the present study, we used contact guidance to control the interactions between the material topography, the cells and the ECM for three different tissues, namely vascular media, corneal stroma and dermal tissue. Using a specific surface topography on an elastomeric material, we observed the orientation of a first cell layer along the patterns in the material. Orientation of the first cell layer translates into a physical cue that induces the second cell layer to follow a physiologically consistent orientation mimicking the structure of the native tissue. Furthermore, secreted ECM followed cell orientation in every layer, resulting in an oriented self-assembled tissue sheet. These self-assembled tissue sheets were then used to create 3 different structured engineered-tissue: cornea, vascular media and dermis. We showed that functionality of such structured engineered-tissue was increased when compared to the same non-structured tissue. Dermal tissues were used as a negative control in response to surface topography since native dermal fibroblasts are not preferentially oriented in vivo. Non-structured surfaces were also used to produce randomly oriented tissue sheets to evaluate the impact of tissue orientation on functional output. This novel approach for the production of more complex 3D tissues would be useful for clinical purposes and for in vitro physiological tissue model to better understand long standing questions in biology.
  • Publication
    Mechanical properties of tissue-engineered vascular constructs produced using arterial or venous cells
    (Mary Ann Liebert, Inc. Publishers, 2011-04-02) Guillemette, Maxime.; Germain, Lucie; Galbraith, Todd; Larouche, Danielle; Aubé, David; Marcoux, Hugo; Hayward, Cindy Jean; Bourget, Jean-Michel; Auger, François A.; Gauvin, Robert
    There is a clinical need for better blood vessel substitutes, as current surgical procedures are limited by the availability of suitable autologous vessels and suboptimal behavior of synthetic grafts in small caliber arterial graft (<5 mm) applications. The aim of the present study was to compare the mechanical properties of arterial and venous tissue-engineered vascular constructs produced by the self-assembly approach using cells extracted from either the artery or vein harvested from the same human umbilical cord. The production of a vascular construct comprised of a media and an adventitia (TEVMA) was achieved by rolling a continuous tissue sheet containing both smooth muscle cells and adventitial fibroblasts grown contiguously in the same tissue culture plate. Histology and immunofluorescence staining were used to evaluate the structure and composition of the extracellular matrix of the vascular constructs. The mechanical strength was assessed by uniaxial tensile testing, whereas viscoelastic behavior was evaluated by stepwise stress-relaxation and by cyclic loading hysteresis analysis. Tensile testing showed that the use of arterial cells resulted in stronger and stiffer constructs when compared with those produced using venous cells. Moreover, cyclic loading demonstrated that constructs produced using arterial cells were able to bear higher loads for the same amount of strain when compared with venous constructs. These results indicate that cells isolated from umbilical cord can be used to produce vascular constructs. Arterial constructs possessed superior mechanical properties when compared with venous constructs produced using cells isolated from the same human donor. This study highlights the fact that smooth muscle cells and fibroblasts originating from different cell sources can potentially lead to distinct tissue properties when used in tissue engineering applications.
  • Publication
    Microstructured human fibroblast-derived extracellular matrix scaffold for vascular media fabrication
    (John Wiley & Sons, Inc, 2016-04-28) Guillemette, Maxime.; Tondreau, Maxime; Laterreur, Véronique; Germain, Lucie; Miville-Godin, Caroline; Ruel, Jean; Mounier, Maxence; Tremblay, Catherine; Labbé, Raymond; Bourget, Jean-Michel; Veres, Teodor; Auger, François A.; Gauvin, Robert
    In the clinical and pharmacological fields, there is a need for the production of tissue-engineered small-diameter blood vessels. We have demonstrated previously that the extracellular matrix (ECM) produced by fibroblasts can be used as a scaffold to support three-dimensional (3D) growth of another cell type. Thus, a resistant tissue-engineered vascular media can be produced when such scaffolds are used to culture smooth muscle cells (SMCs). The present study was designed to develop an anisotropic fibroblastic ECM sheet that could replicate the physiological architecture of blood vessels after being assembled into a small diameter vascular conduit. Anisotropic ECM scaffolds were produced using human dermal fibroblasts, grown on a microfabricated substrate with a specific topography, which led to cell alignment and unidirectional ECM assembly. Following their devitalization, the scaffolds were seeded with SMCs. These cells elongated and migrated in a single direction, following a specific angle relative to the direction of the aligned fibroblastic ECM. Their resultant ECM stained for collagen I and III and elastin, and the cells expressed SMC differentiation markers. Seven days after SMCs seeding, the sheets were rolled around a mandrel to form a tissue-engineered vascular media. The resulting anisotropic ECM and cell alignment induced an increase in the mechanical strength and vascular reactivity in the circumferential direction as compared to unaligned constructs.