Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge
|Authors:||Beauchamp, Chantal Jeanne; Lévesque, Gabriel; Prévost, Danielle; Chalifour, François-Philippe|
|Abstract:||Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited.
Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo.
Two Gram-negative N2-fixing isolates were identified as Pseudomonas. At 20 C, both isolates revealed that N2-fixing activity
was higher than that of three arctic Pseudomonas strains. Their N2-fixing activity was found to occur between 18 and 25 C, a pattern
that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N2-fixing activity after carbohydrate
amendments both with and without inoculation of a N2-fixing isolate. These results suggest that DPS composts support
N2-fixing bacteria and that N2-fixing activity is dependent on a usable carbohydrate source.
2005 Elsevier Ltd. All rights reserved.|
Keywords: Dinitrogen-fixing bacteria; Compost; Paper sludge; Glucose; Pseudomonas balearica; Pseudomonas putida
|Document Type:||Article de recherche|
|Issue Date:||1 May 2006|
|Open Access Date:||16 March 2016|
|This document was published in:||Bioresource technology, Vol. 97 (8), 1002–1011 (2006)|
|Collection:||Articles publiés dans des revues avec comité de lecture|
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