Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge

Authors: Beauchamp, Chantal Jeanne; Lévesque, Gabriel; Prévost, Danielle; Chalifour, François-Philippe
Abstract: Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas. At 20 C, both isolates revealed that N2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N2-fixing activity was found to occur between 18 and 25 C, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N2-fixing activity after carbohydrate amendments both with and without inoculation of a N2-fixing isolate. These results suggest that DPS composts support N2-fixing bacteria and that N2-fixing activity is dependent on a usable carbohydrate source. 2005 Elsevier Ltd. All rights reserved.
Keywords: Dinitrogen-fixing bacteria; Compost; Paper sludge; Glucose; Pseudomonas balearica; Pseudomonas putida
Document Type: Article de recherche
Issue Date: 1 May 2006
Open Access Date: 16 March 2016
Document version: AM
Permalink: http://hdl.handle.net/20.500.11794/350
This document was published in: Bioresource technology, Vol. 97 (8), 1002–1011 (2006)
https://doi.org/10.1016/j.biortech.2005.04.041
Elsevier
Alternative version: 10.1016/j.biortech.2005.04.041
Collection:Articles publiés dans des revues avec comité de lecture

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