RAD sequencing reveals within-generation polygenic selection in response to anthropogenic organic and metal contamination in North Atlantic eels

Authors: Laporte, Martin; Pavey, Scott; Rougeux, Clément; Pierron, Fabien; Lauzent, Mathilde; Budzinski, Hélène; Labadie, Pierre; Geneste, Emmanuel; Couture, Patrice; Baudrimont, Magalie; Bernatchez, Louis
Abstract: Measuring the effects of selection on the genome imposed by human-altered environ- ment is currently a major goal in ecological genomics. Given the polygenic basis of most phenotypic traits, quantitative genetic theory predicts that selection is expected to cause subtle allelic changes among covarying loci rather than pronounced changes at few loci of large effects. The goal of this study was to test for the occurrence of poly- genic selection in both North Atlantic eels (European Eel, Anguilla anguilla and Ameri- can Eel, A. rostrata ), using a method that searches for covariation among loci that would discriminate eels from ‘control’ vs. ‘polluted’ environments and be associated with specific contaminants acting as putative selective agents. RAD-seq libraries resulted in 23 659 and 14 755 filtered loci for the European and American Eels, respec- tively. A total of 142 and 141 covarying markers discriminating European and Ameri- can Eels from ‘control’ vs. ‘polluted’ sampling localities were obtained using the Random Forest algorithm. Distance-based redundancy analyses (db-RDAs) were used to assess the relationships between these covarying markers and concentration of 34 contaminants measured for each individual eel. PCB153, 4 0 4 0 DDE and selenium were associated with covarying markers for both species, thus pointing to these contami- nants as major selective agents in contaminated sites. Gene enrichment analyses sug- gested that sterol regulation plays an important role in the differential survival of eels in ‘polluted’ environment. This study illustrates the power of combining methods for detecting signals of polygenic selection and for associating variation of markers with putative selective agents in studies aiming at documenting the dynamics of selection at the genomic level and particularly so in human-altered environments.
Keywords : distance-based redundancy analysis, landscape genomics, polygenic selection, RAD sequencing, Random Forest algorithm
Document Type: Article de recherche
Issue Date: 12 December 2015
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/349
This document was published in: Molecular Ecology, Vol. 25 (1), 219–237 (2016)
https://doi.org/10.1111/mec.13466
John Wiley & Sons, Inc.
Alternative version: 10.1111/mec.13466
Collection:Articles publiés dans des revues avec comité de lecture

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