Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation
|Authors:||Baillon, Lucie; Oses, Jennifer; Pierron, Fabien; Du Colombier, Sarah Bureau; Caron, Antoine; Normandeau, Éric; Lambert, Patrick; Couture, Patrice; Labadie, Pierre; Budzinski, Hélène; Dufour, Sylvie; Bernatchez, Louis; Baudrimont, Magalie|
|Abstract:||Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined.
Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered
as critically endangered of extinction. Pollution is one of the putative causes for the collapse of this
species. Among their possible effects, contaminants gradually accumulated in eels during their somatic
growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to
potential toxic events in gonads. The aim of this study was to investigate the effects of organic and inorganic
contaminants on the gonad development of wild female silver eels. Female silver eels from two
sites with differing contamination levels were artificially matured. Transcriptomic analyses by means
of a 1000 candidate gene cDNA microarray were performed on gonads after 11 weeks of maturation to
get insight into the mechanisms of toxicity of contaminants. The transcription levels of several genes, that
were associated to the gonadosomatic index (GSI), were involved in mitotic cell division but also in gametogenesis.
Genes associated to contaminants were mainly involved in the mechanisms of protection
against oxidative stress, in DNA repair, in the purinergic signaling pathway and in steroidogenesis, suggesting an impairment of gonad development in eels from the polluted site. This was in agreement with
the fact that eels from the reference site showed a higher gonad growth in comparison to contaminated
Keywords: Anguilla anguilla Silver stage Contamination Gonad development Transcriptomic
|Document Type:||Article de recherche|
|Issue Date:||6 July 2015|
|Open Access Date:||Restricted access|
|This document was published in:||Chemosphere, Vol. 139, 303–309 (2015)|
|Collection:||Articles publiés dans des revues avec comité de lecture|
All documents in CorpusUL are protected by Copyright Act of Canada.