Variants of a genomic island in Aeromonas salmonicida subsp. salmonicida link isolates with their geographical origins

Authors: Emond-Rheault, Jean-Guillaume; Vincent, Antony T.; Trudel, Mélanie V.; Brochu, Francis; Boyle, Brian; Tanaka, Katherine H.; Attéré, Sabrina A.; Jubinville, Éric; Loch, Thomas P.; Winters, Andrew; Faisal, Mohamed; Frenette, MichelDerome, NicolasCharette, Steve
Abstract: Aeromonas salmonicida subsp. salmonicida is a fish pathogen. Analysis of its genomic characteristics is required to determine the worldwide distribution of the various populations of this bacterium. Genomic alignments between the 01-B526 pathogenic strain and the A449 reference strain have revealed a 51-kb chromosomal insertion in 01-B526. This insertion (AsaGEI1a) has been identified as a new genomic island (GEI) bearing prophage genes. PCR assays were used to detect this GEI in a collection of 139 A. salmonicida subsp. salmonicida isolates. Three forms of this GEI (AsaGEI1a, AsaGEI1b, AsaGEI2a) are now known based on this analysis and the sequencing of the genomes of seven additional isolates. A new prophage (prophage 3) associated with AsaGEI2a was also discovered. Each GEI appeared to be strongly associated with a specific geographic region. AsaGEI1a and AsaGEI2a were exclusively found in North American isolates, except for one European isolate bearing AsaGEI2a. The majority of the isolates bearing AsaGEI1b or no GEI were from Europe. Prophage 3 has also a particular geographic distribution and was found only in North American isolates. We demonstrated that A. salmonicida subsp. salmonicida possesses unsuspected elements of genomic heterogeneity that could be used as indicators to determine the geographic origins of isolates of this bacterium.
Keywords : Bacteria, Genomics-functional genomics-comparative genomics; Furunculosis; Aeromonas salmonicida; Fish pathogen; Genomic island; Geographical distribution
Document Type: Article de recherche
Issue Date: 22 November 2014
Open Access Date: 16 March 2016
Document version: AM
Permalink: http://hdl.handle.net/20.500.11794/312
This document was published in: Veterinary microbiology, Vol. 175 (1), 68–76 (2015)
https://doi.org/10.1016/j.vetmic.2014.11.014
Elsevier Scientific Pub. Co.
Alternative version: 10.1016/j.vetmic.2014.11.014
Collection:Articles publiés dans des revues avec comité de lecture

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