RAD-QTL mapping reveals both genome-level parallelism and different genetic architecture underlying the evolution of body shape in Lake Whitefish (Coregonus clupeaformis) species pairs

Authors: Laporte, Martin; Rogers, Sean Michael; Dion-Côté, Anne-Marie; Normandeau, Éric; Gagnaire, Pierre-Alexandre; Dalziel, Anne C.; Chebib, Jobran; Bernatchez, Louis
Abstract: Parallel changes in body shape may evolve in response to similar environmental conditions, but whether such parallel phenotypic changes share a common genetic basis is still debated. The goal of this study was to assess whether parallel phenotypic changes could be explained by genetic parallelism, multiple genetic routes, or both. We first provide evidence for parallelism in fish shape by using geometric morphometrics among 300 fish representing five species pairs of Lake Whitefish. Using a genetic map comprising 3438 restriction site-associated DNA sequencing single-nucleotide polymorphisms, we then identified quantitative trait loci underlying body shape traits in a backcross family reared in the laboratory. A total of 138 body shape quantitative trait loci were identified in this cross, thus revealing a highly polygenic architecture of body shape in Lake Whitefish. Third, we tested for evidence of genetic parallelism among independent wild populations using both a single-locus method (outlier analysis) and a polygenic approach (analysis of covariation among markers). The single-locus approach provided limited evidence for genetic parallelism. However, the polygenic analysis revealed genetic parallelism for three of the five lakes, which differed from the two other lakes. These results provide evidence for both genetic parallelism and multiple genetic routes underlying parallel phenotypic evolution in fish shape among populations occupying similar ecological niches.
Keywords : Adaptive radiation, Parallel evolution, Fish body shape, Geometric morphometrics, Genotyping-by-sequencing.
Document Type: Article de recherche
Issue Date: 21 May 2015
Open Access Date: 16 March 2016
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/307
This document was published in: G3 Genes Genomes Genetics, Vol. 5 (7), 1481-1491 (2015)
https://doi.org/10.1534/g3.115.019067
Genetics Society of America
Alternative version: 10.1534/g3.115.019067
Collection:Articles publiés dans des revues avec comité de lecture

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