Practical ring-opening strategy for the sequence determination of cyclic peptides from one-bead-one-compound libraries

Authors: Liang, Xinxia; Girard, Anick; Biron, Éric
Abstract: The use of cyclic peptides in one-bead-one-compound libraries is limited by difficulties in sequencing hit compounds. Lacking a free N-terminal amine, such peptides cannot be sequenced by the Edman degradation approach, and complex fragmentation patterns are obtained by tandem mass spectrometry. To overcome this problem, we designed an alternative approach introducing a methionine residue within the macrocycle and as a linker to allow simultaneous ring-opening and release from the resin upon treatment with cyanogen bromide. The methionine linker was inverted relative to the peptide chain to allow the synthesis of cyclic peptides anchored by a lysine side chain and to avoid the presence of two C-terminal homoserine lactones on the released linear peptides. After MALDI-TOF MS/MS analysis, the peptides released from a single bead were sequenced manually and with a de novo sequencing software. The strategy described herein is compatible with commonly used amino acids and allows sequencing of cyclic peptides in one-bead-one-compound libraries, thus reducing the need for encoding. -- KEYWORDS: cyclic peptide libraries, one-bead-one-compound libraries, peptide macrocycles, ring-opening, peptide sequencing
Document Type: Article de recherche
Issue Date: 9 October 2013
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/197
This document was published in: ACS Combinatorial Science, Vol. 15 (10), 535-540 (2013)
https://doi.org/10.1021/co4000979
American Chemical Society
Alternative version: 10.1021/co4000979
24016186
Collection:Articles publiés dans des revues avec comité de lecture

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