Aerosolization of mycobacteria and legionellae during dental treatment : low exposure despite dental unit contamination

Authors: Dutil, SteveVeillette, MarcMériaux, Anne; Lazure, Louis; Barbeau, JeanDuchaine, Caroline
Abstract: Dental unit waterlines (DUWL) support growth of a dense microbial population that includes pathogens and hypersensitivity-inducing bacteria, such as Legionella spp. and non-tuberculous mycobacteria (NTM). Dynamic dental instruments connected to DUWL generate aerosols in the work environment, which could allow waterborne pathogens to be aerosolized. The use of the real-time quantitative polymerase chain reaction (qPCR) provides a more accurate estimation of exposure levels compared with the traditional culture approach. Bioaerosol sampling was performed 13 times in an isolated dental treatment room according to a standardized protocol that included four dental prophylaxis treatments. Inhalable dust samples were taken at the breathing zone of both the hygienist and patient and outside the treatment room (control). Total bacteria as well as Legionella spp. and NTM were quantified by qPCR in bioaerosol and DUWL water samples. Dental staff and patients are exposed to bacteria generated during dental treatments (up to 4.3 E + 05 bacteria per m3 of air). Because DUWL water studied was weakly contaminated by Legionella spp. and NTM, their aerosolization during dental treatment was not significant. As a result, infectious and sensitization risks associated with legionellae and NTM should be minimal.
Document Type: Article de recherche
Issue Date: 25 July 2007
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/12896
This document was published in: Environmental Microbiology, Vol. 9 (11), 2836–2843 (2007)
https://doi.org/10.1111/j.1462-2920.2007.01395.x
Blackwell Science
Alternative version: 10.1111/j.1462-2920.2007.01395.x
17922766
Collection:Articles publiés dans des revues avec comité de lecture

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