Crown ether helical peptides are preferentially inserted in lipid bilayers as a transmembrane ion channels
|Authors:||Savoie, Jean-Daniel; Otis, François; Bürck, Jochen; Ulrich, Anne; Voyer, Normand|
|Abstract:||Oriented circular dichroism was used to study the alignment crown ether-modified peptides. The influence of different N- and C-functionalities was assessed using at variable peptide:lipid ratios from 1:20 to 1:200. Neither the functionalities nor the concentration had any major effect on the orientation. The alignment of the 21-mer peptides was also examined with lipid membranes of different bilayer thickness. The use of synchrotron radiation as light source allowed the study of peptide:lipid molar ratios from 1:20 to 1:1000. For all conditions studied, the peptides were found to be predominantly incorporated as a transmembrane helix into the membrane, especially at low peptide concentration, but started to aggregate on the membrane surface at higher peptide:lipid ratios. The structural information on the preferred trans-bilayer alignment of the crown ether functional groups explains their ion conductivity and is useful for the further development of membrane-active nanochemotherapeutics.|
|Document Type:||Article de recherche|
|Issue Date:||27 July 2015|
|Open Access Date:||22 November 2016|
|This document was published in:||Peptide Science, Vol. 104 (4), 427–433 (2015)|
Wiley Periodicals, Inc.
|Collection:||Articles publiés dans des revues avec comité de lecture|
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